Banca de QUALIFICAÇÃO: VIVIANNE LAYS RIBEIRO CAVALCANTI

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : VIVIANNE LAYS RIBEIRO CAVALCANTI
DATE: 29/09/2023
TIME: 09:00
LOCAL: https://meet.google.com/mmr-mvuk-zha
TITLE:

ANTI-CANCER ACTIVITY OF LECTINS EXTRACTED FROM PHOTOSYNTHETICIZING MICRO-ORGANISMS

KEY WORDS:

Microalgae; anticancer; colon-rectal cancer; mechanism of action

PAGES: 45
BIG AREA: Ciências Biológicas
AREA: Bioquímica
SUBÁREA: Bioquímica dos Microorganismos
SUMMARY:

Cancer is the name given to a group of more than 100 diseases that are
disordered cell growth is common, which can even
invade other tissues, causing a high mortality rate, thus
established as an important public health problem. In the search for
new molecules with biotechnological potential, several prospecting studies
have been carried out with macromolecules from photosynthetic organisms,
such as microalgae. Lectins from marine organisms demonstrate
relevant biotechnological potential, in which several biological activities are
described in the literature. This work aimed to evaluate the antitumor activity of
lectin isolated from the microalga Chlorella vulgaris (CvL) on cancer cells
colorectal (CCR), HT-29. CvL was extracted, purified and concentrated to
carrying out tests in cell culture. The cytotoxic effect of CvL was evaluated
through cell viability assay using the salt colorimetric method
tetrazolium MTT. The cell line was exposed to concentrations of 50 to 5
µg.ml-1 of CvL for 72h. The IC50 was determined and this value was used as
pattern for clonogenic activities, migration, cell cycle and type of death
(apoptosis and necrosis).The results showed a 92% reduction in the viability of HT-29 in
concentrations of 50, 35 and 25 e µg.ml-1 (IC50 = 21.5 µg.ml-1). CvL was able to
killing CRC cells by both early and late apoptosis, inhibited
colony formation in 100%, in addition to dragging the cell cycle between phases
G2 and S, interrupting the progress of mitosis. Therefore, it is possible to infer
that CvL is a potential chemotherapeutic agent against CRC.

COMMITTEE MEMBERS:
Externa à Instituição - CLAUDENISE CALDAS DA SILVA DANTAS VIEGAS - UPE
Interna - MARIA TACIANA CAVALCANTI VIEIRA SOARES
Externa à Instituição - MARLLYN MARQUES DA SILVA
Presidente - RAQUEL PEDROSA BEZERRA