Banca de DEFESA: AMANDA LINS BISPO MONTEIRO

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : AMANDA LINS BISPO MONTEIRO
DATE: 30/08/2023
TIME: 14:00
LOCAL: on line
TITLE:

ANALYSIS OF THE CHEMICAL COMPOSITION AND COATING FOR THE PRESERVATION OF FRUITS POST-HARVEST WITH PROPOLIS OF THE STINGLESS BEE Trigona spinipes (ARAPUÁ)

KEY WORDS:

Stingless bees, propolis, coating, postharvest fruits

PAGES: 153
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUMMARY:

Meliponines or stingless bees, constitute a wide group of species distributed all over the world and are used in meliponiculture, a rational breeding practice, identified as an important source of income for small producers due to the commercialization of products. These species are divided into two tribes: Meliponini and Trigonini, and produce geopropolis and propolis, respectively. Propolis is one of the most popular products and consists of a complex resinous matrix produced by bees by collecting plant exudates and adding wax. In this sense, the objective of this work was to analyze the chemical constituents of Trigona spinipes bee propolis collected from September 2018 to June 2021 in the São Francisco Valley region, totaling 15 samples, in addition to evaluating the content of phenolic compounds and flavonoids, the anti-radical capacity, antimicrobial activity and propose the formulation of a coating based on propolis for preserving fruits in the post-harvest process with evaluations of mass loss, firmness, colorimetry, pH variation, titratable acidity, and total solids. The evaluation of the propolis constituents was obtained by obtaining the ethanolic extract, later partitioned into different polarities with the solvents: hexane, ethyl acetate, and methanol: water (1:1). For better identification of the compounds, two samples were submitted to solid phase extraction (SPE) and hydrolysis, acetate fraction and hexane fraction of TS-C7 and TS-C8, respectively. And then the indicated composition through techniques such as HPLC, UPLC-DAD-qTOF-MS/MS, GC/MS, IR, APT, 1H, and two-dimensional techniques such as COZY, HMBC, and HSQC. Through GC/MS and APT E 1H NMR analysis of isolated propolis mixtures, it was possible to identify the main classes of compounds: alkanes, alkenes, esterified triterpenoids, esterified fatty acids, and anacardic acids. The analysis by UPLC-DAD-qTOF-MS/MS allowed the identification of 24 compounds such as flavones, flavanones, and flavonoids in general, and with the help of HPLC-DAD verified the similarity between propolis and the species Mimosa tenuiflora (jurema preta), indicating that the bee has the habit of visiting the species. The content of phenolics and flavonoids varied between samples and their fractions, indicating that the time of collection influences the chemical composition, and consequently the antiradical and antimicrobial activities. The coated fruits showed a significant difference over time between the treatment and the control, mainly in the following parameters: acidity, pH, peel, and pulp color analysis. The weight loss analysis increased during the experiment for both treatments, being lower in the coated fruits. The differences and effectiveness presented in the study between the control group and the treatment indicates that the success of the coating is directly related to the chemical composition of the coating added with propolis from the Trigona spinipes bee.

COMMITTEE MEMBERS:
Presidente - TANIA MARIA SARMENTO DA SILVA
Interna - TATIANA SOUZA PORTO
Externa à Instituição - BÁRBARA VIVIANA DE OLIVEIRA SANTOS
Externa à Instituição - AYALA NARA PEREIRA GOMES
Externo à Instituição - KRISTERSON REINALDO DE LUNA FREIRE