Banca de QUALIFICAÇÃO: MATHEUS CANDIDO FEITOSA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : MATHEUS CANDIDO FEITOSA
DATE: 21/08/2023
TIME: 13:00
LOCAL: google meet
TITLE:

USE OF CELL THERAPY USING MESENCHYMAL STEM CELLS IN THE TREATMENT OF CORNEAL ULCER IN AN EXPERIMENTAL MODEL: CLINICAL EVALUATION, HISTOLOGICAL AND IMMUNOHISTOCHEMISTRY ANALYSIS

KEY WORDS:

Ophthalmology, Treatment, Dogs, Clinical signs.

PAGES: 50
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Clínica e Cirurgia Animal
SUMMARY:

Studies using mesenchymal stem cells (MSC) in veterinary ophthalmology demonstrate a reduction in the early inflammatory reaction of local corneal cells and innate immune cells through paracrine mechanisms, in addition to benefits such as reduction of ocular discharge, conjunctival hyperemia and demonstrates that when there is its use in the acute phase of trauma, there is less local angiogenesis, less chance of opacity, in addition to better and faster healing of the corneal lesion, in addition to presenting lower and significant levels of formation of tumor necrosis factor alpha (TNFα) , a group of cytokines capable of causing cell death. Regenerative medicine using cell therapy arrives bringing new possibilities for treatments in veterinary medicine and ophthalmology, thinking about patients and improving the quality of life of those affected by diseases, bringing possibilities little applied in veterinary therapy, which when compared to the huge series of corneal injury, arising the urgency to observe it in a better applied way. After administration of 0.5x106 cells/mL in the subconjunctival sac of the eye with ulcer of the experimental models, with the first group (G1 – N=8) applied 1 hour after injury, the second group (G2 – N=8), applied cells with 24 hours after injury and the control groups, containing 8 animals for the G1 group, 8 animals for the G2 group and 8 animals for comparison in a general control group, totaling 40 animals. A reduction of ulcers was observed within 48 hours in all animals that received stem cell application (16 animals), regardless of the time of injury, with 1 hour of MSC application or 24 hours of cell application. In addition to the reduction of ulcerative lesions, it was noted that there was no additional process to injury or recovery process such as: conjunctival discharge, opacity, neovascularization, blepharospasm or pigmentation, throughout the process of evaluation days.

COMMITTEE MEMBERS:
Presidente - ANISIO FRANCISCO SOARES
Externo ao Programa - 385059 - FABRICIO BEZERRA DE SA - UFRPEExterno ao Programa - 2279588 - MARCELO WEINSTEIN TEIXEIRA - UFRPEExterna à Instituição - MICHELE ANDRADE DE BARROS - PUC-CAMPINAS