Campylobacter spp. is considered an important gastrointestinal bacterial pathogen due to the implications for industrial poultry in terms of public health. Campylobacter jejuni infection is one of the most common causes of Guillain-Barré syndrome and may also be related to hemolytic uremic syndrome in humans. The consumption of undercooked chicken meat or food for consumption that has been in contact with raw chicken is the most common source of infection for humans, since the intestines of healthy birds can be colonized by this bacteria, leading to contamination of the carcasses. However, the increase in poultry production has led to the continued use of antibiotics in the production chain and this practice has led to the selection of resistant bacteria, including Campylobacter. Therefore, the objective of this research was to evaluate the contamination of chicken carcasses sold in supermarkets by Campylobacter and to detect the thermophilic species of importance for public health: C. jejuni, C. coli and C. lari, genes related to virulence and resistance, determine the antimicrobial resistance profile of the isolates and evaluate the response of the isolates to the action of enterocins. Samples of liver, gizzard and skin from different regions of the carcass (chilled and frozen) were evaluated. The isolation of Campylobacter spp. was in accordance with ISO 10272-2 (2017) guidelines. The isolates were tested by PCR to identify genus, species (C. jejuni, C. coli and C. lari), virulence genes (cdtA, cdtB, cdtC, sodB, dnaJ) and resistance (cmeA, cmeB, cmeC). The assessment of susceptibility to antibiotics was performed using the disk diffusion method, according to EUCAST (2023). The Minimum Inhibitory Concentration (MIC) method was used to determine the antimicrobial activity of enterocins, according to NCCLS (2013). A total of 376 positive isolates were for Campylobacter spp. and 26 (7%) isolates positive for C. jejuni. C. coli and C. lari isolates were not found. The highest frequency of C. jejuni was obtained in chilled carcasses with 88.5% (p < 0.0001), in frozen carcasses a frequency of 11.5% was found. The most frequent location of C. jejuni was the skin of the breast (27%), followed by the skin of the wing (23%), skin of the cloaca (19%), gizzard (15%), liver (8%) and skin of the neck (8%). Gene frequency was determined for: cdtA (11.5%), cdtB (11.5%), cdtC (19%), sodB (34.5%), dnaJ (11.5%), cmeA (15%), cmeB (15%) and cmeC (15%). In 100% (n = 26) of the C. jejuni isolates, multidrug resistance to three or more classes of antimicrobials was found. The antimicrobial activity of enterocins was able to inhibit at least 98.5% of the growth of all C. jejuni isolates. Therefore, it is necessary to establish national Campylobacter monitoring programs in all segments of industrial poultry farming and antimicrobial consumption monitoring systems must be adopted in animal production. In addition, further investigations must be carried out to explore the use of probiotic strains to control this pathogen, as their use has shown promising results.