Banca de DEFESA: LARICE BRUNA FERREIRA SOARES

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : LARICE BRUNA FERREIRA SOARES
DATE: 22/06/2023
TIME: 09:00
LOCAL: Auditório da UFAPE
TITLE:

Evaluation of the antimicrobial potential of plant extracts used in folk medicine in the state of Pernambuco

KEY WORDS:

antioxidant. antimicrobial resistance. natural products. secondary metabolites.

antioxidant. antimicrobial resistance. natural products. secondary metabolites.

 

PAGES: 102
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUMMARY:

In view of the continuous increase in antimicrobial resistance to commercial drugs and the need for new components that can be active against pathogens, the present research work aimed to carry out a study of the chemical composition and evaluation of the antimicrobial potential of plant extracts used in medicine popular, in the state of Pernambuco. Ten plants were collected or acquired from healers in the region and identified, amora (Morus alba), aroeira (Schinus terebinthifolius), cajueiro-roxo (Anacardium occidentale), capim limão (Cymbopogon citratus), gengibre (Zingiber officinale), mandacaru (Cereus jamacaru), pitanga (Eugenia uniflora), sambacaitá (Hyptis pectinata), unha-de-gato (Uncaria tomentosa) e uxi-amarelo (Endopleura uchi). Then crude ethanol extracts of these plants were prepared, as well as the aqueous, chloroform and hexane fractions from these extracts. The extracts and fractions were tested against bacteria Staphylococcus aureus, Enterococcus faecalis, Bacillus subtilis, Listeria monocytogenes, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa e Salmonella typhimurium. The isolates were tested for antimicrobial susceptibility profile by Disk Diffusion tests at concentrations of 10 and 20 mg.mL^-1 and Minimum Inhibitory and Bactericidal Concentration (MIC) at concentrations of 0.195 to 25 mg.mL^-1. The content of phenolic and flavonoid compounds was quantified by the Folin-Ciocalteu assay and by the colorimetric method with aluminum chloride, and the antioxidant potential was also evaluated by the 2,2-diphenyl-1-picryl-hydrazyl (DPPH) scavenging methods and 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) from aroeira and pitangueira plants, since they showed greater antimicrobial potential. It was observed that, by the Disk Diffusion test, the crude ethanolic extracts with the highest activity were aroeira and pitangueira, respectively. Among the tested fractions, the aqueous fractions of both plants showed the greatest inhibition potential, acting against all gram-positive and gram-negative strains tested. It was possible to identify which mastic crude extract showed better activity for all strains tested, especially for B. subtilis, E. faecalis, L. monocytogenes, S. aureus, E. coli and K. pneumoniae with MIC values ranging from 0.78 to 1.56 mg.mL^-1 which demonstrates its moderate activity against these strains. Quantitative analysis of the content of total phenolic compounds reveals their greater presence in the aqueous fractions than the crude extracts, since the content of total flavonoids was more expressive for the crude extracts, for both plants. The results of the present study suggest that for most gram-positive and gram-negative strains tested, S. terebinthifolius shows moderate activity, highlighting a possible potential for its performance as a broad-spectrum antimicrobial. In turn, E. uniflora, despite having a lower inhibition potential by the MIC test when compared to S. terebinthifolius, still shows biological potential, which can encourage further research. Both plants show a high content of phenolic compounds and an excellent antioxidant potential.

COMMITTEE MEMBERS:
Presidente - KEILA APARECIDA MOREIRA
Externa à Instituição - ANA CAROLINA DE CARVALHO CORREIA
Externo à Instituição - JOSÉ ERICK GALINDO GOMES
Externa à Instituição - ROSANGELA ESTEVAO ALVES FALCAO
Externo à Instituição - VANDERSON BARBOSA BERNARDO