Banca de DEFESA: JULIANA KELLY URTIGAS DE SOUZA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : JULIANA KELLY URTIGAS DE SOUZA
DATE: 16/11/2022
TIME: 08:30
LOCAL: Plataforma Google Meet dia 16/11/2022 às 9h
TITLE:

Potential of peptidases obtained from the latex of Calotropis procera as an immunotherapeutic agent

against Salmonella ssp

KEY WORDS:

Laticifer proteins, Salmonella spp., immunotherapeutic

PAGES: 47
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Medicina Veterinária Preventiva
SPECIALTY: Doenças Infecciosas de Animais
SUMMARY:

Proteases isolated from the latex of the medicinal plant of Calotropis procera have been investigated in different models of inflammation. Considering previous studies that indicated anti-inflammatory properties of a mixture of proteases called LPp2, in this work, a mixture of proteases called LPp3, still little studied, was investigated in an experimental salmonellosis model. Thus, macrophage cultures were exposed to different concentrations of LPp3 and infected with Salmonella enterica Sor. Typhimurium. In another infection model, Swiss mice were infected intraperitoneally with S. Typhimurium and then treated (intravenously) with LPp3. The results showed that LPp3 has no direct antibacterial action against Salmonella in vitro and was unable to increase the cell viability of infected macrophages. In the in vivo assays, after 6 h of infection, animals treated with LPp3 (10 mg/kg) had a higher bacterial load in the spleen and liver compared to untreated control (PBS) or Dexamethasone-administered groups. Treatments with LPp3 (1, 5 and 10 mg/kg) inhibited the recruitment of leukocytes to the infectious site after Salmonella inoculum in the peritoneal cavity. However, gene expression levels of inflammatory cytokines measured in the spleen, such as TNF-alpha and IL1-beta, were significantly increased in animals treated with 10 mg/kg relative to infected/untreated animals. It was concluded that LPp3, as already demonstrated for LPp2, is rich in enzymes with strong anti-inflammatory action, which could explain the lower elimination of bacteria at higher doses.

BANKING MEMBERS:
Interno - ANISIO FRANCISCO SOARES
Interno - JOSE VITOR MOREIRA LIMA FILHO
Interno - VALDEMIRO AMARO DA SILVA JUNIOR
Externo à Instituição - MÁRCIO VIANA RAMOS - UFC
Externa à Instituição - BETTY MANCEBO DORVIGNY