Banca de DEFESA: SÂMARA DA SILVA GOMES

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : SÂMARA DA SILVA GOMES
DATE: 10/10/2022
TIME: 08:00
LOCAL: Remoto via Google Meet
TITLE:

Toxicity of the pesticide mixtures sulfentrazone, fipronil and carbendazim on zebrafish (Danio rerio) embryos and larvae

KEY WORDS:

Fish, Pesticides Mixture, Epiboly, Teratogenicity, Behavioral effects.

PAGES: 135
BIG AREA: Ciências Biológicas
AREA: Fisiologia
SUMMARY:

Brazil is the largest consumer of agrochemicals in the world. Contamination of surface waters by pesticides is commonly associated with a combination of substances rather than a single compound because several active ingredients are commonly applied simultaneously to crops and/or a variety of crops that typically use the same watersheds. The current evaluation of the toxic effects of these pesticides is predominantly based on single substances, so the ecological relevance may be underestimated. Given this, the present study aimed to investigate the effects of the combined use of a herbicide, Sulfentrazone, an insecticide, Fipronil, and a fungicide, Carbendazim. To achieve this goal, epiboly, teratogenicity in zebrafish embryos, and behavioral changes in zebrafish larvae were analyzed by performing the Thigmotaxis, Sensitive touch, Optomotor response, Bouncing balls, and Exploratory activity tests. The three isolated pesticides were teratogenic for zebrafish embryos. In the epiboly analysis, the individual pesticides reduced the epiboly percentage, and the mixtures caused no significant differences. However, there were significant changes in the performance of larvae exposed to the pesticides and their mixtures in three behavioral tests (Optomotor response, Bouncing balls, and Exploratory activity). In the optomotor response and exploratory activity tests, it was possible to observe the synergistic effect of the binary mixtures. In the bouncing ball test, all exposed groups showed a significant difference compared to the control group. Through our results, we observed that the toxicity of the mixtures differs depending on the endpoints analyzed and that the behavioral tests were sensitive to evaluate the interaction of the pesticide mixtures used in this research. Therefore, studies that seek to understand the effects of pesticide mixtures are important, because they will help to understand the effects more realistically and more closely to what happens in the environment. Because the additive effects of these mixtures are still poorly understood and make their toxicological evaluation difficult.

BANKING MEMBERS:
Presidente - PABYTON GONCALVES CADENA
Interno - ANISIO FRANCISCO SOARES
Interna - ***.982.884-** - MARIA ADELIA BORSTELMANN DE OLIVEIRA - UFRPE
Externo ao Programa - 1088089 - PIERRE CASTRO SOARES - UFRPEExterno à Instituição - EDUARDO PACHECO RICO