Banca de QUALIFICAÇÃO: GABRIELA HÉMYLIN FERREIRA MOURA

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : GABRIELA HÉMYLIN FERREIRA MOURA
DATE: 23/05/2022
TIME: 08:00
LOCAL: Plataforma on-line
TITLE:

Occurrence of anti-Toxoplasma gondii antibodies (IgG) and DNA of protozoa of the Sarcocystidae family in wild birds and mammals from the states of Pernambuco and Rio Grande do Norte

KEY WORDS:

Toxoplasma gondii; Isospora spp; Sarcocystis spp.; wild animals; Caatinga; Atlantic forest

PAGES: 70
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Medicina Veterinária Preventiva
SPECIALTY: Doenças Infecciosas de Animais
SUMMARY:

The objective of this study was to evaluate the occurrence of anti-Toxoplasma gondii antibodies in armadillos (Euphractus sexcinctus), collared peccaries (Collared pecary) and agoutis (Dasyprocta leporina), totaling 55 free-living animals from the Caatinga biome using the MAT technique. In addition, protozoa of the Sarcocystidae family were also researched in 71 wild birds from the Pernambuco Wild Animal Triage Center (CETAS-Tangara) and 25 free-living birds from the Caatinga biome in the state of Rio Grande do Norte, totaling 96 animals belonging to 41 species. Brain fragments were subjected to molecular diagnosis by nested PCR for the 18s rDNA gene of Apicomplexa protozoa, followed by genetic sequencing. In the first study, antibodies were detected in 7/29 (24.13%) giant armadillos, 6/16 (37.5%) collared peccaries and absence of antibodies in the agoutis. In the second study, the gene was detected in 25% (24/96) of the analyzed samples, and from these it was possible to perform DNA sequencing of 14 samples, confirming three genera of protozoa of the Sarcocystidae family: Isospora spp., Sarcocystis spp . and Toxoplasma gondii in samples from eight bird species (Amazona aestiva, Coereba flaveola, Egretta thula, Paroaria dominicana, Sporophila nigricollis, Cariama cristata, Columbina talpacoti, Crypturellus parvirostris). The occurrence of coccidia of the Sarcocystidae family in wild birds demonstrates the need to intensify research in this area for the conservation of wild birds in the Caatinga and Atlantic Forest biomes

BANKING MEMBERS:
Externa à Instituição - DEBORA ROCHELLY ALVES FERREIRA
Externa à Instituição - RENATA PIMENTEL BANDEIRA DE MELO - UFRPE
Presidente - 2118675 - RINALDO APARECIDO MOTA