Evaluation of the lethal and subletal effects of oil from the accident that occurred on the pernambuco coast in marine invertebrate
crude oil, Tisbe biminiensis, bioassays, environmental disaster, oil contamination
The objective of this study was to evaluate the effect of crude oil that contaminated the Brazilian coast. For this, ecotoxicological tests were carried out using the benthic copepod Tisbe biminiensis as a test organism. The bioassays lasted one month, with a weekly water change. It was used 0.5 g of oil collected in 4 different beaches of the Pernambuco coast (Enseada dos Corais, Xaréu, Cupe and Paiva) and 40 mL of filtered sea water and basic food for macerated aquarium fish. At the end of the exposure period, the content was fixed and stained with Rose Bengal for later counting and determination of endpoints (number of copepods, population number and percentage of females). Stereomicroscopic observations revealed spots on the individuals, due to this fact, a new bioassay was performed for photographic record with the same conditions as the previous one, using only oil from two beaches (Enseada dos Corais and Paiva), without the use of dye, for better results. microscope view. there was a significant decrease in the number of copepods (in the groups exposed to the oil collected in Xaréu, Enseada dos Corais and Paiva) and in the population number (in all the oil samples tested). In addition, there was a feminization of the population, since there was a significant increase in the proportion of females in oil samples collected at Paiva beach and Enseada dos Corais. Through the photographs we verified that the oil was ingested, part of the ingested oil contaminates the animal, and the other part is fragmented and leaves in the fecal pellets, becoming available for other organisms. Therefore, we conclude that the oil that contaminated the Brazilian coast has a high toxic power for the tested organism.