Banca de DEFESA: DENNY PARENTE DE SÁ BARRETO MAIA LEITE

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : DENNY PARENTE DE SÁ BARRETO MAIA LEITE
DATE: 17/02/2022
TIME: 09:00
LOCAL: PPGBA UFRPE - meet
TITLE:

Identification and phenotypic and genotypic resistance profile of Staphylococcus aureus isolated from veterinarians, guardians, animals and hospital environment in the city of Recife, Brazil

KEY WORDS:

Antimicrobials. blaZ . canines. Cats. nor(A). OS-MRSA

PAGES: 93
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Medicina Veterinária Preventiva
SPECIALTY: Doenças Infecciosas de Animais
SUMMARY:

Antimicrobial resistance is one of the main threats to public health. Staphylococcus aureus resistant to different classes of antimicrobials is a priority for the implementation of preventive measures. Therefore, the objective of the study was to identify the bacterium in dogs and cats, in humans with close contact with these animals (guardians and veterinarians), veterinary instruments and veterinary hospital surfaces environment, to evaluate the genotypic and phenotypic profile of antimicrobial resistance. Bacterial recovery was performed using sterile swabs obtained in the second semester of 2021. 20 humans, 13 animals, 14 ambulatory surfaces, 7 veterinarians’ stethoscopes and 8 veterinarians cell phones samples at the Veterinary Hospital of the Federal Rural University of Pernambuco were collected. Isolation was performed on Agar Salt Mannitol and preliminary identification by Gram stain and catalase test. Subsequently, the species S. aureus was confirmed, and its genotypic profile was investigated by polymerase chain reaction. To determine the phenotypic profile of resistance, the disk-diffusion method was performed. Ten S. aureus isolates were recovered in 35% (7/20) of humans, 10% (1/10) of dogs, 33% (1/3) of cats and 7.14% (1/14) of inanimate surfaces. The phenotype mecA-positive oxacillin-sensitive S. aureus (OS-MRSA) was identified in a feline sample. The genotypic profile of bacterial specimens indicated resistance to different classes of antimicrobials, with a higher frequency of resistance to beta-lactams and quinolones, respectively, 80% (8/10) were holders of the blaZ gene and 90% (9/10) of the nor(A). In the phenotypic evaluation, all samples were resistant to penicillin and 80% (8/10) were resistant to erythromycin. Additionally, induced resistance by erythromycin to clindamycin were verified in the same 8 samples resistant to erythromycin (80%). Finally, the presence of genetic determinants of antimicrobial resistance with the phenotypic profile in S. aureus requires attention. The transfer of these resistance genes between bacteria may occur. Also, the risk of antimicrobial therapy failure in staphylococcal infections is possible.

BANKING MEMBERS:
Presidente - 1803550 - TATIANA SOUZA PORTO
Interno - 1652122 - JOSE WILTON PINHEIRO JUNIOR
Externo à Instituição - JOSÉ GIVANILDO DA SILVA