Banca de DEFESA: LUCAS VITOR BATISTA RODRIGUES

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : LUCAS VITOR BATISTA RODRIGUES
DATE: 21/02/2024
TIME: 15:00
LOCAL: Auditório do Departamento de Química
TITLE:

METABOLOMIC STUDY OF Piper marginatum WHICH OCCURS IN PERNAMBUCO FOR SUSTAINABLE CONTROL OF Plutella xylostella.

KEY WORDS:

Brassica, Croweacin, (E)-isoelemicin, Exalatacin

PAGES: 89
BIG AREA: Ciências Exatas e da Terra
AREA: Química
SUBÁREA: Química Orgânica
SPECIALTY: Química dos Produtos Naturais
SUMMARY:

Piper marginatum is a plant known for presenting a high production of EO in addition to having secondary metabolites with insecticidal activities. However, when the plant is subjected to biotic and abiotic factors, the chemical composition of these EOs can change. This variation in chemical composition can affect the biological properties and insecticidal activit that P. marginatum oil has on Plutella xylostella. The P. xylostella is an agricultural pest that occurs in Brassica species, causing damage to farmers, leading to the total loss of Brassica production. Currently, this pest is controlled using synthetic insecticides. However, the indiscriminate use of these insecticides leads to ecosystem imbalance, soil contamination, the emergence of resistant generations and causing neurodegenerative diseases in applicators. In this study, individuals of P. marginatum were cultivated under different stress conditions, in order to map their chemical profile using metabolomic techniques and verify their anti-sensitivity potential on P. xylostella larvae. Twenty-seven specimens of P. marginatum were divided into 9 groups. These groups were subjected to stressful conditions, exposure to UV radiation, application of elicitors, mechanical predation, water deficit, water scarcity and different soil pH. The EOs of the control group and stressed P. marginatum were obtained by hydrodistillation. GC-MS analysis of different stress conditions revealed croacin, (E)-isoelemycin, (Z)-isoelemycin, exalatacin and (E)-caryophyllene as the main constituents. These results show that under different stress conditions P. marginatum presented qualitative and quantitative variations in its chemical composition of EO. With the aim of evaluating the insecticidal potential of the different EOs of P. marginatum, a residual contact test was carried out against P. xylostella larvae. The residual contact bioassay revealed a mean lethal concentration (LC50) of 0.34 μL/mL. The EO from P. marginatum leaves was 8 time more toxic to the pest than the commercial insecticide Azamax®. These results indicate that P. marginatum EO has the potential to become a product of natural origin for the control of important pests in Brassica cultivation.

COMMITTEE MEMBERS:
Presidente - CLAUDIO AUGUSTO GOMES DA CAMARA
Interno - JOAO RUFINO DE FREITAS FILHO
Interno - MARCILIO MARTINS DE MORAES
Externo à Instituição - RODOLFO RODRIGUES DA SILVA - UFRPE