Banca de QUALIFICAÇÃO: JOSE EUDES DA SILVA DE OLIVEIRA

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : JOSE EUDES DA SILVA DE OLIVEIRA
DATE: 22/09/2023
TIME: 09:00
LOCAL: Auditório do Departamento de Química
TITLE:

Development of electrochemical sensors for simultaneous detection and quantification of methylated nucleic acid compounds

KEY WORDS:

DNA Adducts, 7-Methylguanine, 5-Methylcytosine, 3-Methyladenine, Carbon, Voltametry.

PAGES: 82
BIG AREA: Ciências Exatas e da Terra
AREA: Química
SUBÁREA: Química Analítica
SPECIALTY: Eletroanalítica
SUMMARY:

The main objective of this work was to investigate the electrocatalytic oxidation of 7-methylguanine (7-mGua) and 5-methylcytosine (5-mCyt) in different aqueous electrolytes, on the surface of the cathodically pre-treated boron-doped diamond electrode (red-BDDE) and for 3-methyladenine (3-mAde) on the anodically pre-treated glassy carbon electrode (oxi-GCE). Furthermore, we sought to develop a simple, sensitive and selective electrochemical method for the simultaneous quantification of methylated bases, 7-mGua and 5-mCyt, using the BDDE-network and differential pulse voltammetry (DPV), in the presence of possible interferences and for future applications in hydrolyzed DNA samples. An electroanalytical method using DPV and oxi-GCE was also investigated for detection and quantification of 3-mAde. This approach represents a significant innovation in DNA methylation analysis, showing promise for future applications in medical research and diagnosis.

COMMITTEE MEMBERS:
Presidente - SEVERINO CARLOS BEZERRA DE OLIVEIRA
Interno - MARCILIO MARTINS DE MORAES
Interno - RAMOM RACHIDE NUNES
Externo à Instituição - VAGNER BEZERRA DOS SANTOS - UFPE