Banca de DEFESA: MARIA EDUARDA BEZERRA COUTINHO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : MARIA EDUARDA BEZERRA COUTINHO
DATE: 30/07/2025
TIME: 08:30
LOCAL: Auditório do Departamento de Química da UFRPE
TITLE:

DEVELOPMENT OF GREEN ANALYTICAL METHODOLOGIES FOR THE QUANTIFICATION OF ARTIFICIAL COLORS IN FOOD PRODUCTS USING DIGITAL IMAGES WITH SMARTPHONE

KEY WORDS:

Artificial colors; RGB; Food safety; Smartphones.

PAGES: 84
BIG AREA: Ciências Exatas e da Terra
AREA: Química
SUBÁREA: Química Analítica
SPECIALTY: Análise de Traços e Química Ambiental
SUMMARY:

The use of artificial colors is common due to their ability to improve the appearance and attractiveness of food. However, excessive consumption can cause adverse effects, such as hypersensitivity and hyperactivity, especially in children, and monitoring is essential to ensure the health of consumers. In this context, two studies focused on rapid analytical methods based on direct digital image acquisition (DIA) were developed, aligned with the principles of green analytical chemistry. In Study 1, the Allura Red AC dye (E129), widely present in candies, was determined through the optimization of sample preparation based on the reference method of the Adolfo Lutz Institute using molecular absorption spectrophotometry in the UV-Vis region, and also by developing a low-cost colorimetric method using a smartphone and portable photometer (DIA-RD). The DIA-RD method enabled the simultaneous acquisition of the analytical curve (2.00x10-4 to 2.00x10-2 mg mL-1) and candy samples, using the RGB model and multivariate analysis by PLS in the REDGIM® software. The accuracy of the UV-Vis method ranged from 77.33% ± 1.53 to 98.35% ± 0.07, with a limit of quantification (LOQ) of 2.26x10-6 mg mL-1 and a limit of detection (LOD) of 7.47x10-7 mg mL-1. For DIA-RD, the accuracy ranged from 78.04% ± 1.42 to 98.42% ± 0.06, with a LOQ of 1.67x10-4 mg mL-1 and a LOD of 5.51x10-5 mg mL-1. In ten candy samples, the concentrations of E129 were below the limits set by national and international legislation. In Study 2, DIA-RD was applied in the quantification of Brilliant Blue FCF (E133) and Tartrazine Yellow (E102) dyes in alcoholic and non-alcoholic beverages, both individually and in mixtures, with linear curves between 1.00x10-4 to 9.90x10-3 mg mL-1. In the UV-Vis reference method, the accuracy between the curves containing the isolated and mixed dyes ranged from 96.06 ± 7.34x10-3 to 110.12 ± 3.06x10-2, with LOQ from 1.24x10-5 to 3.29x10-4 mg mL-1 and LOD from 4.10x10-6 to 1.08x10-4 mg mL-1. For DIA-RD, the accuracy between the curves ranged from 93.72 ± 1.27x10-2 to 110.67 ± 4.16x10-2, with LOD ranging from 1.78x10-5 to 4.95x10-5 mg mL-1 and LOQ from 5.76x10-3 to 5.40x10-5 mg mL-1. The E133 and E102 dyes were quantified in commercial alcoholic and non-alcoholic beverages at concentrations below the compliance limits established by national and international legislation. The developed methods proved to be effective, accessible, and environmentally friendly, with in situ applications via smartphone, highlighting the potential of the DIA-RD method in the quality control of food products following the principles of green analytical chemistry.

COMMITTEE MEMBERS:
Presidente - JANDYSON MACHADO SANTOS
Interno - RAMOM RACHIDE NUNES
Externo à Instituição - VAGNER BEZERRA DOS SANTOS - UFPE