Banca de DEFESA: JULIANE CUTRIM MARQUES

CHEMICAL, PHYSICOCHEMICAL ANALYSIS AND ANTIRADICAL ACTIVITY OF THE LEAF, BARK AND PULP OF THE SPECIES Attalea maripa

Attalea maripa, antiradical activity, physicochemical analysis

The present work brought studies related to Attalea maripa (Aubl.) Mart, a palm tree
native to the North, Northeast and Midwest regions of Brazil, located in the Amazon biome. O
objective was to trace the chemical profile (leaf and pulp), evaluate the antiradical activity
of the parts of the palm tree (leaf, bark and pulp) and the physical-chemical analysis (pulp and leaf). To the
samples were submitted to techniques such as high performance liquid chromatography (HPLC),
gas chromatography coupled to mass spectrometry (GC-MS), magnetic resonance
(NMR) and infrared (IR) spectroscopy, in addition to antiradical studies by
DPPH• and ABTS•+ method and assays for total phenolics and flavonoids. The compound profile
was obtained through ethanol extracts and hexane partitions, ethyl acetate and
methanol:water (1:1). The partitions were submitted to column chromatography, highlighting
for the methanol:water fraction of the leaf and the methanolic extract of the pulp. through techniques
using HPLC, GC/MS, IR and 1 H and 13 C NMR, the presence of polysaccharides and acids is assumed
fatty acids, respectively, in the leaf and pulp fractions, identifying alkanes, alkenes and acids
carboxylic acids through IV analyses. The phenolic and flavonoid contents were shown to be
significant in ethanolic extracts and in the acetate part of the leaf, bark and pulp and it can be
observe that they influence the antioxidant capacity of the tests made of DPPH and ABTS. You
results of the centesimal composition analysis of the pulp revealed 41.38% of moisture, pH
4.96, 9.7% total soluble solids, 1.2% total titratable acidity, 5.90% ash, 28.19
% lipids, 4.55% proteins, 7.94% total sugars, 2.13% reducing sugars and
5.52% of non-reducing sugars. The leaf showed pH 6.29, 4.96% ash and 7.94%
lipids. A colorimetric test characterized the flesh color as light and vibrant yellow.
Chemical techniques, from profiling to identification, will be refined by two-dimensional approaches and
new analyzes with more precise chromatographic methods for future articles.
The detection of a substance in mass spectrometry opens up research possibilities

in toxicity and preservation. This study contributes as a small portion in the literature
on the species Attalea maripa.