Banca de QUALIFICAÇÃO: ALINE DE MELO VIEIRA

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : ALINE DE MELO VIEIRA
DATE: 29/02/2024
TIME: 14:00
LOCAL: Em ambiente virtual (Google meet)
TITLE:

DEVELOPMENT OF AN ANALYTICAL METHOD FOR THE QUANTIFICATION OF ILLICIT DRUGS IN SURFACE WATERS IN THE METROPOLITAN REGION OF RECIFE/PE

KEY WORDS:

Illicit drugs, surface water, solid phase extraction, analytical method validation and GC/MS analysis.

PAGES: 76
BIG AREA: Ciências Exatas e da Terra
AREA: Química
SUBÁREA: Química Analítica
SPECIALTY: Análise de Traços e Química Ambiental
SUMMARY:

Illicit drugs (ID) and their metabolites are considered emerging contaminants since, after consumption, they are partially metabolized and excreted mainly through urine and can reach wastewater treatment plants (WWTPs). However, the efficacy of these stations in removing such substances is often limited, resulting in their presence in aquatic ecosystems. In this context, a solid phase extraction (SPE) method was developed and validated, followed by analysis by gas chromatography-mass spectrometry (GC-MS). So, a 2³ factor design was carried out to find suitable conditions for extracting 6 analytes from different classes of DI, including cocaine (COC), benzoylecgonine (BE), cocaethylene (CEE), methamphetamine (MANF), 3,4- methylenedioxymethamphetamine (MDMA), and the cannabis metabolite, 11-nor-9-carboxy-Δ⁹-tetrahydrocannabinol (THC-COOH). The parameters evaluated in the design were sample volume, cartridge washing and conditioning/elution solvent. The optimum conditions were defined, and the experiment was conducted using 200 mL of surface water sample, loaded onto a Strata-X cartridge (200 mg; 6mL), without the washing step and using a conditioning/elution solvent of 3 mL of MeOH followed by 3 mL of EtOAc. The analytical response was evaluated through the recovery of the analytes for each experiment. The method was then validated following the Standard Practices for Method Validation in Forensic Toxicology guide. After validation, the method was applied to investigate the presence of DI in surface water from the RMR, thus 18 real surface water samples, 9 of which were collected from the Tejipió River and 9 from the Beberibe River, in two different periods. It was possible to determine COC and BE in 100% of the samples, CEE was found in 22% and THC-COOH was determined in 38% of the samples. The results obtained in this study highlight a greater presence of COC and BE in the Beberibe and Tejipió rivers, especially during carnival festivities.

COMMITTEE MEMBERS:
Presidente - JANDYSON MACHADO SANTOS
Interno - RAMOM RACHIDE NUNES
Externo à Instituição - RAMON ALVES DE OLIVEIRA PAULA - UFMG
Externa à Instituição - GIOVANA ANCESKI BATAGLION - UFAM