Banca de QUALIFICAÇÃO: ANA FLÁVIA BARBOSA DE OLIVEIRA

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : ANA FLÁVIA BARBOSA DE OLIVEIRA
DATE: 29/09/2023
TIME: 14:00
LOCAL: Em ambiente virtual (Google meet)
TITLE:

INVESTIGATION OF THE CONSUMPTION OF PSYCHOACTIVE DRUGS IN THE RECIFE AND OLINDA CARNIVAL THROUGH SEWAGE EPIDEMIOLOGY

KEY WORDS:

Wastewater-based epidemiology, method development, psychoactive drugs, solid-phase extraction, gas chromatography, mass spectrometry.

PAGES: 87
BIG AREA: Ciências Exatas e da Terra
AREA: Química
SUBÁREA: Química Analítica
SPECIALTY: Análise de Traços e Química Ambiental
SUMMARY:

In the context of psychoactive drug consumption estimates, wastewater-based epidemiology has stood out as a rapid, precise, and cost-effective approach compared to traditional methods. With the aim of providing a new perspective on psychoactive drug consumption in the Metropolitan Region of Recife (RMR) and investigating the influence of festive events and weekends on trends in psychoactive drug consumption, this study sought to develop a solid-phase extraction (SPE) method, followed by gas chromatography-mass spectrometry analysis, for six analytes with distinct chemical properties, which were: cocaine (COC) and its main metabolites (benzoylecgonine (BE) and cocaethylene (CEE)), methamphetamine (MAMP), 3,4-methylenedioxymethamphetamine (MDMA), and the cannabis metabolite, 11-nor-9-carboxy-Δ9-tetrahydrocannabinol (THC-COOH). To achieve this, a full factorial experimental design (23) was conducted to identify the optimal conditions for SPE sample preparation. These conditions were defined as using 100 mL of raw sewage sample, washing the Strata-X extraction cartridge (200 mg, 6 mL) with 6 mL of ultrapure water, and conditioning/eluting with 6 mL of MeOH, resulting in satisfactory recoveries of all analytes in the range of 70% to 120%. This demonstrated efficiency by enabling the successful inclusion of THC-COOH extraction in simultaneous analysis with other multiple analytes of different chemical polarities. The developed method was validated following the guidelines of the Standard Practices for Method Validation in Forensic Toxicology and complied with the established validation specifications and limits. The applicability of the method was demonstrated by analyzing 28 raw wastewater samples collected over 7 days during the carnival and 7 days during a typical week at the two main wastewater treatment plants (WWTPs) in the RMR: WWTP Cabanga, located in the city of Recife, and the WWTP Peixinhos, located in Olinda. The preliminary data presented here show that it was possible to quantify COC, BE, and THC-COOH in all samples, while CEE was quantified in 28% of them. For all analytes, the highest concentrations and daily loads were recorded during the carnival period, with a notable increase also observed during the weekends of a typical week without festive events. These results highlight the influence of festive events and weekends on psychoactive drug consumption in the RMR. As a perspective, the data obtained so far, in conjunction with population estimates, will enable the retrocalculation of consumption estimates, expressed in mg/day/1000 inhabitants. Thus, this study has contributed to a more comprehensive understanding of trends in psychoactive drug consumption in part of the RMR.

COMMITTEE MEMBERS:
Presidente - JANDYSON MACHADO SANTOS
Interno - ANDRE FERNANDO LAVORANTE
Interno - MARCILIO MARTINS DE MORAES
Externa à Instituição - GIOVANA ANCESKI BATAGLION