Banca de DEFESA: FÁBIA MARTINS DA SILVA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : FÁBIA MARTINS DA SILVA
DATE: 29/02/2024
TIME: 08:00
LOCAL: AUDITÓRIO
TITLE:

BIOPROSPECÇÃO DE PLANTAS DA RESERVA DE DOIS IRMÃOS (RECIFE-PE) NA BUSCA DE NOVAS MOLÉCULAS COMO PROTÓTIPOS PARA DESENVOLVIMENTO DE ANTIBIÓTICOS E RETROVIRAIS

KEY WORDS:

Reserve of twoBrothers, Bioprospecting, Ethanolic extracts, antimicrobial potential

PAGES: 97
BIG AREA: Ciências Exatas e da Terra
AREA: Química
SUMMARY:

The Reserva de Dois Irmãos located in the city of Recife Pernambuco, has a plant
biodiversity that directs a peculiar attention to an area that can be considered a green
pharmacy in the middle of the capital of Pernambuco. The objective of this work was to
evaluate the antimicrobial potential of the species that occur in the Ecological Reserve
of Dois Irmãos in search of new molecules that are candidates for antibiotics. Ethanol
extracts from 30 species were obtained for evaluation of antimicrobial activity, and
chemical profile by CG-MS and 1H NMR. Thus, the first 21 species that presented
antimicrobial activity for two extracts presented IMC values of 39 μg/mL, the extract of
the species Cordyline terminalis for the fungus Penicilium sp and the species Cordyline
terminalis for the fungus C. albicans. The nine other plant species were analyzed by
the biological activity IMC-IC50. Of which extracts Inga laurina, Apeiba tirbouboul,
Mormodica charantia and Ipomoea asarifolia showed strong potential against
Escherichia coli. The extracts Coffea arabica, Guarea guidonia and Morus nigra
showed moderate activity against the bacterium Klebsiela pneumoneae. Candida
albicans observing the cell growth curve showed high antimicrobial resistance for all
extracts tested. In the extracts Inga laurina, Apeiba tirboubou,,Ipomea asarifolia ,
Mormodica charantia, Plumeria pudica and Morus nigra had similar profiles of 1H NMR
with characteristic signs of lipid, terpene, fatty acids and steroids hydrogens. The
species of Ochorama pyramidale 31% of flavanol Afzechina. Coffea arabica 47%
caffeine and in the species Guarea guidonia a sesquiterpene bicycle derived from the
selinesans with 29.9% and 20.95% of the curcuphenol compound. The monitored
fractionation of prudish Plumeria resulted in fraction D obtained better antimicrobial
potential against Escherichia coli. The fractionation of the crude ethanol extract of
paubrasilia echinata leaves allowed the isolation and structural elucidation by 1H and
13C NMR of diterpene called Echinalide H. molecular docking of the seven diterpenes
tested, two were active in the models (integrase and reverse transcriptase), only the
compound 6β-hydroxy-18-vouacapaneoic acid B derivative showed activity against
integrase with 52.42% of probable antiviral activity

COMMITTEE MEMBERS:
Externo à Instituição - ADALBERTO MANOEL DA SILVA
Presidente - CLECIO SOUSA RAMOS
Interno - JOAO RUFINO DE FREITAS FILHO
Externo ao Programa - 3022573 - JUCLEITON JOSE RUFINO DE FREITAS - nullExterna à Instituição - QUEILA PATRÍCIA DA SILVA BARBOSA FREITAS