INSECTICIDAL ACTIVITY OF BINARY MIXTURES OF THE ESSENTIAL OIL OF Melaleuca leucadendra WITH LYCARIN A AND PIPLARTIN ON Plutella xylostella
Insecticidal activity, Piper cyrtopodom, Piper tuberculatum. Synergism, E-nerolidol.
The Plutella xylostella, popularly known as the diamondback moth, is the main pest that attacks oilseed species of the Brassicaceae family, such as cabbage, cauliflower, cabbage and others. These crops are responsible for great movement in the world economy and their interruption causes irreparable economic damage. The mains form of pest controls are through chemicals controls, with the application of commercial insecticides based on different chemical classes, such as carbamates, pyrethroids, organophosphates, among others.. However, the indiscriminate use of these insecticides often ends up affecting non-target organisms, in addition to contaminating the environment. As an alternative to commercial insecticides, products of natural origin such as essential oils, extracts and pure compounds have been gaining ground in the formulation of botanical insecticides. In the present work, mixtures were formulated between the essential oil of the leaves of Melaleuca leucadendra with the compounds licarin A and piplartine isolated from the leaves of Piper cyrtopodom and Piper tuberculatum, respectively. The essential oil was obtained by hydrodistillation and its analysis by Gas Chromatography coupled to Mass Spectrometry (GC-MS) revealed E-nerolidol (97.68%) as the major constituent. Both the essential oil (LC50 = 0.17μL/mL), licarin A (LC50 = 18.38μL/mL) and piplartine (LC50 = 7.84μL/mL) were toxic to P. xylostella. The binary mixture (07:03) of M. leucadendra oil with licarin A resulted in a synergistic mixture with larvicidal toxicity of LC50 = 0.13 μL/mL. The embryocidal toxicity of this mixture was performed, showing an average lethal concentration of 0.002 μL/mL. The mixture of piplartine and M. leucadendra resulted in four synergistic mixtures, in the proportions 09:01, 05:05, 04:06 and 01:09. In this way, the mixtures between the essential oil of M. leucadendra and the isolated compounds of lycarin A and piplartine proved to be active and promising as a formulation for the control of the pest Plutella xylostella.