Banca de DEFESA: JOSE PAULINO DA SILVA NETO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : JOSE PAULINO DA SILVA NETO
DATE: 03/03/2023
TIME: 14:00
LOCAL: Departamento de Química
TITLE:

DEVELOPMENT AND TOXICOLOGICAL EVALUATION OF A NEW OXIDOVANADIUM(4+) COMPLEX CONTAINING TAURINE AND ASCORBATE

KEY WORDS:

diabetes mellitus, vanadium, oxovanadium, toxicity, taurine, and ascorbate.

PAGES: 75
BIG AREA: Ciências Exatas e da Terra
AREA: Química
SUBÁREA: Química Inorgânica
SPECIALTY: Química Bio-Inorgânica
SUMMARY:

In recent years, vanadium compounds have aroused great interest due to their complex range of biological properties, with high potential for developing drugs with antitumor, antiviral, antioxidant, and mainly antihyperglycemic and/or antidiabetic action.  This work proposed the synthesis and characterization of a new oxovanadium (4+) compound containing taurine and ascorbate as ligands, as well as to evaluate its toxicological potential in vitro and in vivo. The complex obtained was coded as [V^IVO(tau)(asc)], with the minimal formula [V^IVO(C₂H₆NO₃S)(C₆H₇O₆)], whose structure was confirmed by spectroscopy techniques in the infrared region, by the presence of stretching νV=O at 943 cm^-1, ¹H nuclear magnetic resonance (NMR) (δ_(ppm)= 3. 11, 3.32, 3.67-3.60, 4.85) and ¹³C (δ_(ppm): 35.33, 47.35, 62.03, 68.85, 76.16, 117.71, 115.62, and 173.24) and mass spectrometry (ESI-FT-MS) (M= C8H13O10NSV+, m/z=365.97, and (M+1= C₈H₁₄O₁₀NSV⁺, m/z=366.97). The absence of signal in the ⁵¹V NMR aligned with the 8 hyperfine lines in the EPR spectrum, confirms that an oxovanadium (4+) coordination compound with high stability in an aqueous solution was obtained. The oral acute toxicity assay according to OECD protocol 423 determined that the compound fell into category 4 (estimated LD₅₀ between 300 and 2000 mg kg^-1). Biochemical and hematological assays showed no significant difference (p<0.05) in the treated groups compared to the control group, indicating that the compound showed neither nephrotoxicity nor hepatotoxicity at the doses tested. The results indicate that [V^IVO(tau)(asc)] may be a potential candidate antihyperglycemic drug, and with low toxicity. However, the toxicological profile of the compound needs to be further investigated due to the result of LC₅₀ = 1.198 ug/mL against A. salina nauplii.

COMMITTEE MEMBERS:
Externa à Instituição - LÍGIA CRISTINA MONTEIRO GALINDO
Interno - JOAO RUFINO DE FREITAS FILHO
Presidente - MONICA FREIRE BELIAN