Banca de DEFESA: SABRINA CRUZ DE SOUZA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : SABRINA CRUZ DE SOUZA
DATE: 24/02/2023
TIME: 15:00
LOCAL: Video Conferência
TITLE:

PHYTOCHEMICAL STUDY AND ACARICIDAL PROPERTY OF THE ESSENTIAL OIL, EXTRACT AND PURE COMPOUND OF Piper solmsianum ON Tetranychus urticae

KEY WORDS:

Acetylcholinesterase. Chemical composition. Germacrene D. Grandisina

PAGES: 68
BIG AREA: Ciências Exatas e da Terra
AREA: Química
SUBÁREA: Química Orgânica
SPECIALTY: Química dos Produtos Naturais
SUMMARY:

The species P. solmsianum belonging to the Piperaceae family is found in the form of bushes in fragments of the Atlantic Forest, popularly known as pariparoba or caapeba. Although few works are reported in the literature regarding chemical and biological studies, phytochemical investigations point to several bioactive properties for the P. solmsianum species, such as antimicrobial activity, fungicide and insecticide potential. Pest control measures in agriculture often involve the use of synthetic insecticides, however the continued and inappropriate use of these products has caused toxic effects to non-target organisms and contributed to the emergence of resistant pests. In view of this, new strategies and/or alternative technologies to commercial insecticides are being developed, such as insecticides formulated based on extracts and essential oils from plants. The scarcity of chemical and biological studies for the essential oil and leaf extracts of P. solmsinaum motivated the performance of the phytochemical study, as well as the evaluation of the acaricidal, antioxidant and cytotoxic properties against Artemia salina. Leaves of P. solmsianum were collected in the city of Curitiba, Paraná, Brazil. The essential oil was obtained via hydrodistillation using a modified Clevenger-type apparatus and the chemical composition determined via gas chromatography coupled to mass spectrometry. Chemical characterization of the extract and pure compound was performed using high performance liquid chromatography. The acaricidal activity was evaluated through the residual contact test on Tetranychus urticae and the AChE enzyme inhibition test. The biological model selected for the evaluation of cytotoxicity was the test against Artemia salina. The essential oil presented sesquiterpenes as the main chemical class. The main compounds identified were germacrene D (17.18 %), β-caryophyllene (11.30 %) and δ-cadinene (10.67 %). The essential oil for controlling T. urticae showed an LC₅₀ = 58.8 μL.mL^-1 and did not show AChE enzyme inhibition activity. The extract showed better antioxidant capacity for the free radical DPPH˙ (EC₅₀ = 65.61 μg.mL^-1) and ABTS˙⁺ (EC₅₀ = 46.10 μg.mL^-1). The cytotoxicity test against Artemia salina nauplii showed that the essential oil has potential toxicity against microcrustaceans. This study showed that the essential oil of P. solmsianum leaves has acaricidal, antioxidant and cytotoxic biological properties.

COMMITTEE MEMBERS:
Presidente - CLAUDIO AUGUSTO GOMES DA CAMARA
Interno - JOAO RUFINO DE FREITAS FILHO
Externa à Instituição - CAROLINA ALVES DE ARAUJO
Externo à Instituição - Everton Vieira da Silva
Externo à Instituição - JOAO PAULO RAMOS DE MELO