Banca de DEFESA: LUÍS EDUARDO GOMES DE HOLANDA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : LUÍS EDUARDO GOMES DE HOLANDA
DATE: 18/08/2022
TIME: 09:00
LOCAL: Remota
TITLE:

DETERMINATION OF LIPID PROFILE BY 1H NMR x GC-MS, PHYSICOCHEMICAL PROPERTIES BY 1 H NMR AND ACTIVITY ANTIMICROBIAL OF OILS EXTRACTED FROM SEEDS OF TROPICAL FRUITS

KEY WORDS:

oils, fatty acids, chemical characterization and antimicrobial potential

PAGES: 140
BIG AREA: Ciências Exatas e da Terra
AREA: Química
SUBÁREA: Química Orgânica
SPECIALTY: Química dos Produtos Naturais
SUMMARY:

Brazil is the third largest fruit producer in the world, with a production of over
45 million tons in 2019, most of this production is consumed by the Brazilian
population, exporting just over 35% of its production. The high production of
agribusiness generates large amounts of waste, producing up to 20.3 million tons of
this byproduct, leaving Brazil among the 10 countries that waste most food in the
world, seeds are considered as one of these waste products coming from the
processing industries of fruit / vegetable, this type of residue could be applied in
various areas such as food, cosmetics and pharmaceuticals adding more value to
this residue In the search for the development of new products of natural origin the
antimicrobial properties against fungi and bacteria, the fixed oils of the species
seeds were evaluated Annona squamosa (pinha), Annona Muricata (graviola),
Annona Atemoya (atemoya), Carica papaya L. (mamão), Cucumis melo (melão),
Cucurbita moschata (abóbora), Citrus reticulata (tangerina), Eugenia uniflora
(pitanga), Malpighia emarginata (acerola), Citrullus lanatus (melancia) which were
chemically characterized by GC-MS, subsequently the percentages of oleic and
linoleic acid were compared with the results obtained by 1 H NMR, where their major
compounds were palmitic, stereatic, oleic and linoleic fatty acids. The
physicochemical characterization was performed by bench protocol according to
Instituto Adolfo Lutz and by 1 H NMR integrals, several quality standards were
selected by 1 H NMR spectroscopy such as: saponification index, iodine and acidity,
such as well the average molecular triacylglycerol weight, total satured fatty acid,
degree of unsaturation (linolenic and oleic acids) and the ratio between olefinic and
aliphatic hydrogens. The antimicrobial inhibitory effects of these oils on
microorganisms were also tested: S.aures, P.aeruginosa, K. pneumoniae, E.
faecalis, C. utilis and M. gypseum. The applied technique was the evaluation of the
Minimum Inhibitory Concentration (MIC). Results of antimicrobial activity ranged
from weak to strong, where the best antimicrobial potential was E. uniflora indicating
activity for all microorganisms, the highest E. uniflora activity was for S.aures, K.
pneumoniae and E. faecalis indicating MIC equal to 39 μg.mL -1 . M. emarginata seed
oil was also active against all microorganisms with MIC ranging from 625 to 1250
μg.mL -1 .

BANKING MEMBERS:
Presidente - 1690582 - CLECIO SOUSA RAMOS
Interno - 1508305 - JOAO RUFINO DE FREITAS FILHO
Interno - 3133784 - MARCILIO MARTINS DE MORAES
Externo ao Programa - 3022573 - JUCLEITON JOSE RUFINO DE FREITAS - UFRPEExterno à Instituição - ADALBERTO MANOEL DA SILVA
Externo à Instituição - ALEX FRANCE MESSIAS MONTEIRO - UFPB