Banca de QUALIFICAÇÃO: CATIANE OLIVEIRA SOUZA

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : CATIANE OLIVEIRA SOUZA
DATE: 29/11/2024
TIME: 10:00
LOCAL: Recife - PE
TITLE:

IMMUNOPHYSIOLOGICAL AND NUTRITIONAL RESPONSES IN AEDES AEGYPTI LINNAEUS (DIPTERA: CULICIDAE) MOSQUITOES EXPOSED TO THE COMPOUNDS TRANS-ANETHOLE, LINALOOL, AND THEIR COMBINATION

KEY WORDS:

Vector, synergism, botanical insecticides, histology, midgut, immune system

PAGES: 70
BIG AREA: Ciências Agrárias
AREA: Agronomia
SUBÁREA: Fitossanidade
SPECIALTY: Entomologia Agrícola
SUMMARY:

The species Aedes aegypti Linnaeus represents a public health threat as the primary vector of arboviruses today. Strategies to contain these diseases focus on reducing vector population density through synthetic pesticides. However, these substances cause environmental damage, are costly, and promote resistance development. Therefore, studying natural insecticides such as essential oils (EO) and derived compounds is necessary to create effective products that maintain mosquito susceptibility without harming the environment. Although terpenes and phenylpropanoids are abundant in EO composition, their immunophysiological and nutritional effects on insects remain poorly understood. This work aimed to evaluate the compounds trans-anethole (phenylpropanoid) and linalool (terpene) as larvicidal agents, both individually and in combination. In larvicidal bioassays, lethal concentrations 50% (LC50) for trans-anethole (68.41 ppm), linalool (240.57 ppm), and their combination (96.43 ppm) were determined. Regarding immune responses, L4 larvae exposed to LC50 values were analyzed for oxidative stress, phenoloxidase activity, melanization, nitric oxide levels, reduced glutathione (GSH), and the content of proteins, lipids, sugars, and glycogen. All treatments induced oxidative stress. The combination of these compounds intensified melanization and other immune responses in the larvae, negatively impacting their nutritional reserves and cellular homeostasis, suggesting that prolonged exposure to this binary mixture may compromise the survival of Ae. aegypti. Future research will investigate the histology and histochemistry of midgut and gonadal cells in larvae and adults of this mosquito. Additionally, the potential pathological changes in these organs and their effects on adult fertility and fecundity will be evaluated. Finally, this thesis aims to provide novel active ingredients for the development of effective and sustainable biopesticides.

COMMITTEE MEMBERS:
Presidente - VALERIA WANDERLEY TEIXEIRA
Externo ao Programa - 2199130 - EMMANUEL VIANA PONTUAL - UFRPEExterna à Instituição - Daniela Maria do Amaral Ferraz Navarro - UFPE