Banca de DEFESA: FERNANDO HENRIQUE BOAVENTURA DE MELO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : FERNANDO HENRIQUE BOAVENTURA DE MELO
DATE: 17/02/2023
TIME: 15:00
LOCAL: Video Conferência
TITLE:

IMMUNOLOGY OF BEES Apis mellifera EXPOSED TO NATURAL AND SYNTHETIC XENOBIOTICS

KEY WORDS:

Decline of Pollinators, Agricultural Pesticides, Immunology, Apoptosis, Cell Proliferation, Oxidative Stress, Nitric Oxide, Phenoloxidase

PAGES: 61
BIG AREA: Ciências Agrárias
AREA: Agronomia
SUBÁREA: Fitossanidade
SPECIALTY: Entomologia Agrícola
SUMMARY:

Given the great importance of Apis mellifera bees as ecological agents, highly associated with humanity, it is essential to identify the causes of their decline. Thus, this work aimed to evaluate the immune system of these pollinators in a comparative way between synthetic and natural pesticides, since the use of these chemical pesticides is pointed out as one of the multifactors that cause the reduction in the population of bees. For this, workers were contaminated via ingestion, for 24 hours, with the CL₅₀ of the insecticide Karate^®, the compound Limonene, and the herbicide Roundup^®. For the Azamax^®, a concentration of 250 mL/100 mL was used, since higher doses were repellent to bees. Then, the workers were used for analysis of apoptosis, cell proliferation, oxidative stress, phenoloxidase enzyme activity and nitric oxide levels. As a result, it was found that all products studied caused midgut cell necrosis, with no evidence of regeneration through cell proliferation. Furthermore, with the exception of Limonene, all xenobiotics underwent oxidative stress, which may explain the observed necrotic process. Although nitric oxide (NO₂) is an important signaling molecule for the recognition of xenobiotics, its excessive production can generate oxidative stress. There was also an increase in phenoloxidase activity in all samples, this enzyme is also involved in the mechanisms of recognition of foreign agents and in the production of melanin. Taken together, the data show the high risk that these bees run with the possibility of coming into contact with these pesticides during their life cycle, whether synthetic or natural. Thus, it is evident that there is a need to test products in isolation.

COMMITTEE MEMBERS:
Presidente - CLAUDIO AUGUSTO GOMES DA CAMARA
Externo ao Programa - 384942 - ALVARO AGUIAR COELHO TEIXEIRA - UFRPEExterno ao Programa - 3133784 - MARCILIO MARTINS DE MORAES - UFRPEExterna à Instituição - GLAUCILANE DOS SANTOS CRUZ