Banca de DEFESA: RAFAELA CASSIANO MATOS

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : RAFAELA CASSIANO MATOS
DATE: 27/02/2025
TIME: 09:00
LOCAL: Online
TITLE:

ANALYSIS OF THE EFFICACY OF DIFFERENT ARTIFICIAL FEEDING METHODS ON THE DEVELOPMENT OF LABORATORY AND FIELD POPULATIONS OF AEDES AEGYPTI (LINNAEUS) AND AEDES ALBOPICTUS (SKUSE) (DIPTERA: CULICIDAE).

KEY WORDS:

Artificial feeding; Vector biology; Aedes albopictus; Aedes aegypti; Membranes; Colony establishment; Field; Laboratory.

PAGES: 45
BIG AREA: Ciências Biológicas
AREA: Parasitologia
SUBÁREA: Entomologia e Malacologia de Parasitos e Vetores
SUMMARY:

The Aedes aegypti and Aedes albopictus mosquitoes are primary and secondary vectors, respectively, of arboviruses such as dengue, Zika, Chikungunya and yellow fever, representing serious threats to global public health. Originally restricted to tropical regions, these vectors have expanded their geographic distribution due to climate change, urbanization and globalization, increasing the risk of epidemics. Therefore, the maintenance and establishment of laboratory colonies have become essential for studies that deepen the knowledge about the biology of these mosquitoes. Artificial methods, such as Hemotek® and Petri dishes with organic or synthetic membranes, have been developed as alternatives to feeding the females of these vectors. Therefore, this study evaluated the effectiveness of these methods in feeding females of Aedes aegypti and Aedes albopictus, analyzing parameters such as fecundity, fertility and hatching rate. Colonies were established from field and laboratory populations, maintained under controlled conditions of temperature, humidity and photoperiod. Five feeding methods were tested: Hemotek® with organic membrane (HT+MO) or Parafilm® (HT+PF), Petri dish with organic membrane (PP+MO) or Parafilm® (PP+PF), and anesthetized mice (CM) as control. For Aedes aegypti, laboratory populations showed higher fecundity and fertility compared to field populations, but the feeding methods did not significantly influence these parameters. In the field, the HT+MO method was more effective, while in the laboratory, HT+PF obtained better results. The hatching rate varied, with the PP+PF method standing out in the field and HT+MO in the laboratory. For Aedes albopictus, the origin of the colonies did not significantly impact the results, but the feeding methods did. HT+MO was comparable to CM in fecundity, while PP+MO was superior in laboratory populations. Both methods with organic membranes were preferred, reflecting the natural behavior of mosquitoes.

COMMITTEE MEMBERS:
Presidente - ***.976.374-** - MARCELO HENRIQUE SANTOS PAIVA - UFPE
Interno - JOSE DIJAIR ANTONINO DE SOUZA JUNIOR
Externa à Instituição - MARIA ALICE VARJAL DE MELO SANTOS