BASELINE OF SUSCEPTIBILITY TO ISOCYCLOSERAM AND DEMOGRAPHIC SUBLETHAL EFFECTS IN Liriomyza sativae Blanchard (DIPTERA: AGROMYZIDAE)
Natural susceptibility, diagnostic concentration, life table, fitness, survival analysis, population biology
The leafminer, Liriomyza sativae, is a polyphagous, cosmopolitan, and multivoltine pest that readily establishes in diverse agricultural systems, making its control difficult once infestation occurs. In Brazil, this species has become a key pest in crops such as melon, and its management relies heavily on chemical control. However, cases of resistance to different insecticides used against the Liriomyza complex have already been reported, reinforcing the need for preventive resistance management strategies, particularly in light of the introduction of new molecules such as isocycloseram. Isocycloseram (IRAC Group 30), commercially available in Brazil since 2024, represents a recent alternative for the control of L. sativae. Nevertheless, information regarding the natural susceptibility of Brazilian populations to this molecule was previously unavailable. Therefore, this study aimed to establish a baseline susceptibility, determine a diagnostic concentration for resistance monitoring, and evaluate the effects of sublethal concentrations (LC₀.₀₁ and LC₁₀) on developmental, reproductive, and population dynamic parameters. The baseline study revealed high homogeneity among the evaluated populations, allowing the establishment of an initial diagnostic concentration of 2 mg/L. Populations resistant to other insecticides exhibited greater tolerance to isocycloseram, although without evidence of cross-resistance. Sublethal exposure assessments using life table analyses demonstrated that the effects of this molecule are dependent on population susceptibility and developmental stage, being more pronounced in susceptible populations, with alterations observed in larval growth, pupal weight, and reproductive parameters. In contrast, more tolerant populations maintained greater demographic stability, reflected in higher R₀ values and shorter developmental times under certain concentrations. It is concluded that establishing a diagnostic concentration and understanding sublethal effects are essential tools for implementing proactive and reactive resistance monitoring strategies, enabling early detection of shifts in L. sativae susceptibility to isocycloseram.