Banca de DEFESA: GLORIA MARIA JARDIM CÂNDIDO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : GLORIA MARIA JARDIM CÂNDIDO
DATE: 31/10/2025
TIME: 10:00
LOCAL: Auditório do Departamento de Química
TITLE:

EFFECTS OF ESSENTIAL OIL OF Citrus nobilis ON THE COPULATIVE BEHAVIOR OF Plutella xylostella (LEPIDOPTERA: PLUTELLIDAE)

KEY WORDS:

Diamondback moth; copulation parameters; residual toxicity; mating; fecundity; fertility; oviposition

PAGES: 50
BIG AREA: Ciências Agrárias
AREA: Agronomia
SUBÁREA: Fitossanidade
SPECIALTY: Entomologia Agrícola
SUMMARY:

Plutella xylostella (Linnaeus) is the main pest of brassicas; resistance to chemical insecticides and associated environmental impacts motivate the search for alternative management strategies. Botanical derivatives, especially essential oils, have been evaluated; however, sublethal effects on behavioral and physiological traits remain less explored. This study aimed to: (i) analyze the chemical composition of the essential oil of Citrus nobilis (Lour.); (ii) evaluate its toxicity against P. xylostella; (iii) assess changes in mating and reproduction after exposure to sublethal concentrations (LC5) of C. nobilis or Azamax®; and (iv) evaluate oviposition preference on treated versus untreated substrates. GC–MS analysis identified limonene (73.21%) and γ-terpinene (17.90%) as the major constituents. Residual toxicity assays estimated LC50 values of 783.75 µg/mL for C. nobilis and 16.54 µg/mL for Azamax. In adults exposed to LC5 via honey solution, C. nobilis increased copulation duration (76.42 ± 4.41 min) but reduced fecundity (37.35 ± 7.43 eggs per female), male longevity (7.42 ± 0.52 days), and female longevity (6.42 ± 0.55 days); similar effects were observed with Azamax. A reduction in mating rate (Azamax) and in successful copulation rate (C. nobilis) was also detected. When larvae were exposed to substrates treated with LC5 of C. nobilis, the emerging adults showed reduced copulation duration (25.85 ± 8.17 min), fecundity (29.00 ± 9.57 eggs per female), and male longevity (8.0 ± 1.70 days), along with decreased mating and copulation rates. Larvae exposed to LC5 of Azamax did not reach adulthood. No difference was observed in oviposition preference between treated and untreated substrates. In conclusion, low concentrations of C. nobilis essential oil negatively affect reproductive and longevity parameters of P. xylostella, potentially influencing population dynamics and highlighting its promise for pest management strategies.

COMMITTEE MEMBERS:
Interno - CLAUDIO AUGUSTO GOMES DA CAMARA
Externa à Instituição - MAYARA FERNANDES DOS SANTOS
Externo à Instituição - WENDEL JOSE TELES PONTES - UFPE