Toxicity of Dillapiole and (E)-Isoelemycin on Planococcus citri and feeding activity of Cryptolaemus montrouzieri Mulsant (Coleoptera: Coccinellidae) in the presence of Piper essential oils
Pest-insect, Predator, Consumption, Toxicity, Ovicidal
The predator Cryptolaemus montrouzieri Mulsant, 1853 (Coleoptera: Coccinellidae) feeds on various insects from the order Hemiptera. Among these, we highlight the citrus mealybug, Planococcus citri Risso, 1813, a pest of economic importance due to the significant damage it causes to different crops. The essential oils of Piper aduncum and Piper marginatum have shown promise in controlling this pest. Therefore, the aim of this study was to evaluate the effects of these oils and their major compounds on the predator C. montrouzieri. To achieve this, the following procedures were carried out: (i) identify the chemical composition of the essential oils of P. aduncum and P. marginatum and isolate the major compounds; (ii) toxicity tests via topical contact and residual contact on P. citri nymphs; (iii) ovicidal potential of the essential oils and their compounds on the eggs of both the prey and the predator; and (iv) prey consumption by C. montrouzieri contaminated with concentrations considered sublethal. The major compounds identified in the oils of P. aduncum and P. marginatum were dilapiol and (E)-isoelemicina, respectively. Apparently, the compounds were more toxic via topical contact than residual contact. (E)-isoelemicina was the most toxic compound in both topical and residual contact, with an estimated LC50 of 0.0039 μg/mL and 0.0053 μg/mL, respectively. The oils of Piper and their respective compounds were more toxic to C. montrouzieri eggs than to P. citri eggs. Prey contaminated with Piper oils did not alter the daily consumption of C. montrouzieri, both in the larval and adult stages. Therefore, the compounds studied here are toxic to P. citri, and the presence of Piper oil in the prey did not affect the consumption of C. montrouzieri. However, further investigations will be needed regarding the selectivity of these products, in order to ensure that these compounds can be used in combination with biological control in the management of P. citri.