Banca de DEFESA: LÍLIAN MARIA CARVALHO FERREIRA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : LÍLIAN MARIA CARVALHO FERREIRA
DATE: 30/07/2024
TIME: 14:00
LOCAL: REMOTO - Videoconferência
TITLE:

IDENTIFICATION AND DIVERSITY ANALYSIS OF INTESTINAL BETA-GLUCOSIDASES FROM THE GH1 FAMILY IN MAJOR SUGARCANE LEPIDOPTERAN PESTS

KEY WORDS:

Diatraea saccharalis, Diatraea impersonatella, Telchin licus, Digestive enzymes, gene expression

PAGES: 65
BIG AREA: Ciências Agrárias
AREA: Agronomia
SUBÁREA: Fitossanidade
SPECIALTY: Entomologia Agrícola
SUMMARY:

Beta-glucosidases (β-glucosidases or BGs) are enzymes that act on carbohydrates and are important in insect-plant interaction and insect xenobiotic metabolism. These enzymes mostly belong to the glycosyl hydrolase family 1 (GH1). They are essential for digestion in lepidopterans, especially when associated with the consumption of cellulosic biomass. Thus, in this study, we aimed to identify and characterize genes encoding enzymes with GH1 domains from the intestinal transcriptomes of Diatraea saccharalis (Fabr., 1794) (Lepidoptera: Crambidae), Diatraea impersonatella (Walker, 1863), and Telchin licus licus (Drury, 1770) (Lep.: Castniidae), using bioinformatics analysis with local and NCBI databases. Of the three species studied, only D. saccharalis has a sequenced and annotated genome deposited in the NCBI. GH1-containing genes were identified in all three species. Based on these data, an initial microsynteny analysis was performed on the gene loci identified in the D. saccharalis genome compared to those of Chilo suppressalis (Lep.: Crambidae) and the model insect Bombyx mori (Lep.: Bombycidae) to verify the conservation of the chromosomal position and context of each GH1 gene found. Subsequently, a phylogenetic analysis was conducted with GH1 sequences from different insect orders, with an emphasis on the order Lepidoptera. Finally, focusing on the species D. saccharalis, we identified GH1 genes that were downregulated when larvae fed on sugarcane, their natural diet. These results were confirmed by RT-qPCR. Finally, we evaluated the effect of the antinutritional compound amygdalin on the expression of GH1 genes to correlate with the results from feeding on sugarcane. Therefore, by identifying and characterizing genes of enzymes expressed in the midgut, which may be involved in cellulose degradation in Lepidoptera, we identified targets with potential biotechnological applications, whether in biofuel production or in the development of tools for controlling sugarcane lepidopteran pests.

COMMITTEE MEMBERS:
Presidente - JOSE DIJAIR ANTONINO DE SOUZA JUNIOR
Externo à Instituição - FERNANDO CAMPOS DE ASSIS FONSECA - IFGO
Externo à Instituição - LEONARDO LIMA PEPINO DE MACEDO - EMBRAPA