Adaptive cost of Liriomyza sativae resistance to spinetoram
Adaptive cost, biological parameters, energy reserves, melon.
Liriomyza sativae Blanchard (Diptera: Agromyzidae) is a key pest of the melon crop, causing between 10% and 15% damage to fruit destined for export. The main method of control is chemical, but biological characteristics of the miner fly facilitate the development of resistance and, consequently, a reduction in effectiveness, as recently observed with spinetoram. This resistance can entail an adaptive cost for the insect. This study compared the biology of susceptible and resistant populations of L. sativae to spinetoram on the hosts Cucumis melo L. (Cucurbitaceae) and Canavalia ensiformis L. (Fabaceae) and assessed the stability of resistance. The resistant population was maintained for ten generations without selection pressure and we carried out concentration-mortality curves to determine the CL50 and resistance ratio in the F1, F2, F4, F6, F8 and F10 generations. The biological parameters were estimated using fifteen couples from both populations and individualized with their respective host plants. The results showed stable resistance to spinetoram. The resistant miner flies developed faster and had larger eggs, regardless of the host, but showed a reduction in longevity under conditions of resistance in both hosts. There was a reduction in R0 and Rm in the C. ensiformis host. Resistance partially affected the insect's cycle, indicating compensation. The finding suggests that the use of C. ensiformis as a refuge area or crop rotation may delay the evolution of resistance.