Banca de DEFESA: MARIA CLARA DA NÓBREGA FERREIRA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : MARIA CLARA DA NÓBREGA FERREIRA
DATE: 27/02/2024
TIME: 09:00
LOCAL: Recife - PE
TITLE:

IMMUNOHISTOCHEMICAL AND IMMUNOLOGICAL RESPONSE OF Aedes aegypti LARVAE (DIPTERA: CULICIDAE) SUBJECTED TO TERPINOLENE AND 1-BUTYL-3,4-METHYLENEDIOXYBENZENE AND THEIR ASSOCIATION

KEY WORDS:

 

Arboviruses, biomolecules, dengue, physiology, immunohistochemistry

PAGES: 75
BIG AREA: Ciências Agrárias
AREA: Agronomia
SUBÁREA: Fitossanidade
SPECIALTY: Entomologia Agrícola
SUMMARY:

Knowledge of the action of botanical compounds is a necessity faced in the control of Aedes aegypti. However, most studies focus on toxicological parameters, with their interaction with the intracellular environment being little explored. Studying the compounds terpinolene and 1-butyl-3,4-methylenedioxybenzene (BMDB), Silva et al. (2016) found its promising insecticidal action on this vector. Given this, the hypothesis was raised that the toxicity of these compounds is related to damage to the intestinal epithelium, impacting its defense mechanisms, and that such damage is more significant and permanent compared to its binary combination, due to its action on multiple target sites. To validate them, their effects were evaluated at cytotoxic (immunohistochemistry - Apoptosis and Cell Proliferation) and immunological (phenoloxicity, nitric oxide and oxidative stress - TBARS and Glutathione Transferase) levels. The LC₅₀ of the isolated compounds were used according to Silva et al. (2016). For the association curve, the LC₅₀ was less toxic than the isolated compounds. The midgut showed histological changes in all treatments when compared to the control, however more severe sequelae such as vacuolization, cell swelling and loss of the peritrophic matrix were expressed in the association. As for apoptosis, only treatments containing BMDB, isolated and associated, showed an increase when compared to the control. In PCNA, larvae from the association showed a lower rate when compared to other treatments. For phenoloxidase and nitric oxide, a reduction in BMDB and the association was found, in relation to the control. In oxidative stress there was no statistical difference in the measurement of glutathione, whereas the TBARS showed a reduction in association. Therefore, it is noteworthy that, among the compounds studied, the binary combination resulting from the association presented the best results resulting from more severe damage. It is worth noting that these results were obtained at CL₅₀, and that higher concentrations, similar to those used in the field, or in different proportions can provide even more expressive and effective effects for the management of this vector.

COMMITTEE MEMBERS:
Presidente - VALERIA WANDERLEY TEIXEIRA
Externa à Instituição - Daniela Maria do Amaral Ferraz Navarro - UFPE
Externa à Instituição - KAMILLA DE ANDRADE DUTRA