Banca de DEFESA: DANIEL LIMA PEREIRA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : DANIEL LIMA PEREIRA
DATE: 31/07/2023
TIME: 14:00
LOCAL: online
TITLE:

MONITORING AND CHARACTERIZATION OF RESISTANCE OF Liriomyza sativae BLANCHARD (DIPTERA: AGROMYZIDAE) TO INSETICIDES

KEY WORDS:

leafminer larva, susceptibility, heritability, inheritance, cross-resistance, metabolism

PAGES: 91
BIG AREA: Ciências Agrárias
AREA: Agronomia
SUBÁREA: Fitossanidade
SPECIALTY: Entomologia Agrícola
SUMMARY:

Several factors contribute to the evolution of insect-pest resistance to a particular insecticide, since the selection pressure due to the indiscriminate use of pesticides, integrated with the type of cultivation system has guided this phenomenon and generated an increase in the frequency of resistant populations, causing failures in the effectiveness of these products in the field. Liriomyza sativae, known as the leaf miner fly, a key pest of several crops, has caused severe damage and losses to producers. The most used method for its management is chemical control. Due to its biological characteristics and the wide range of insecticides used to minimize high infestations of this diptera, reports of control failures are occurring. Insecticides of different modes of action such as cyromazine, cyantraniliprole, and spinetoram have been registered for L. sativae and are applied in an exacerbated manner.  Information about the susceptibility and the basis of resistance of L. sativae is still scarce and this information would help to better manage this pest insect. Thus, this work aims to monitor and characterize the resistance of L. sativae to the main insecticides used in the field for its control, using the methodology proposed by Santos et al. (2020). Populations from different regions of Brazil were collected for this study. The molecules used in monitoring were moderately effective in relation to the diagnostic and recommended concentrations of each insecticide, indicating resistance evolution, by allowing survival of individuals, with mortalities ranging from 3.16% to just over 90%. The populations of Arapiraca and Inhuçu showed the first cases of field resistance to spinetoram. The CL₅₀ values and resistance ratio (RR₅₀) for cyromazine, cyantraniliprole and spinetoram ranged from 5.53 - 86.61 mg i. a./L (RR₅₀ from 1.17 to 15.67 times), 0.12 - 0.91 mg i. a./L (RR₅₀ from 2.22 to 7.45 times) and 0.03 - 44.16 mg i. a./L (RR₅₀ from 3.47 to 1652.17 times), respectively. The selection with spinetoram was maintained at 180 mg i. a./L, showing a CL₅₀ of 88.25 mg i. a./L and an RR₅₀ of 4,219.80-fold. The genetic resistance of L. sativae to spinetoram was autosomal, incompletely recessive, and monofactorial. The discriminatory dose/concentration that makes spinetoram resistance recessive was 60 mg i. a./L and can be used in monitoring. Due to this magnitude of resistance, cross-resistance with spinosad and negative cross-resistance with Indoxacarb, thiamethoxan and cartap were verified. These results contribute to the management of insecticide resistance of L. sativae.

COMMITTEE MEMBERS:
Presidente - HERBERT ALVARO ABREU DE SIQUEIRA
Interno - ***.253.274-** - ELTON LÚCIO ARAUJO - UFERSA
Externo à Instituição - ELISEU JOSÉ GUEDES PEREIRA - UFV
Externo à Instituição - JEFFERSON ELIAS DA SILVA - UFS
Externo à Instituição - TADEU BARBOSA MARTINS SILVA - UESPI