EFFECT OF THERMAL ACCLIMATION ON THE REPRODUCTIVE PHYSIOLOGY AND POPULATION GROWTH OF Tenuisvalvae notata (MULSANT) (COLEOPTERA: COCCINELLIDAE)
Coccinellidae, biological control, acclimatization, reproduction, histochemistry
The lady beetle Tenuisvalvae notata (Mulsant) is an insect that occurs in South America with an optimal temperature range varying from 20-28 ºC, with effects on its reproduction outside this optimal range. Previous studies have shown that T. notata can be acclimated to temperatures outside its optimal range, both low and high up to a limit, which allows insect survival. However, they do not reproduce satisfactorily or totally. Thus, this study investigated how temperature may affect predator insects reproduction, as well as the nutrition and morphology of the gametes of insects subjected to different temperatures. Results showed that lady beetle males were affected by the reduction in temperature, with a delay in sex maturation. Females reared in the laboratory at the control temperature (28 ºC) had higher fecundity in semi-field conditions, followed by those reared at 32 ºC and 18 ºC; this could be related to immature male gametes. Biochemical analyses showed differences in lipids and total sugar contents in male and females gonads, with a reduction in comparison to the control (28 oC). Similar results were found for males, in which testicles of males reared at 32 oC e 18 oC had more cists and empty spaces with no spermatozoa in comparison to the control. There was a reduction in the amount of carbohydrates only in ovarioles of females reared at 18 oC. The amount of total proteins did not differ among treatments. Further studies may focus on aspects of nutrient absorption through the midgut and how this may be related to the trade-off between survival and reproduction in T. notata.