Banca de DEFESA: FELIPE MARINHO COUTINHO DE SOUZA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : FELIPE MARINHO COUTINHO DE SOUZA
DATE: 17/02/2023
TIME: 09:00
LOCAL: Recife
TITLE:

IDENTIFICATION AND CHARACTERIZATION OF MOLECULAR PATTERN RECOGNITION AND β-1,3 ENDOGLUCANASES GENES (GH16 FAMILY) EXPRESSION IN Diatraea saccharalis (LEP.: CRAMBIDAE) DURING ENTOMOPATHOGENS INFECTION

KEY WORDS:

Immune system, GH16 domain, gene expression, Metarhizium anisopliae, Bacillus thuringiensis.

PAGES: 65
BIG AREA: Ciências Agrárias
AREA: Agronomia
SUBÁREA: Fitossanidade
SPECIALTY: Entomologia Agrícola
SUMMARY:

The cuticle is a passive structural barrier in the insect defense system, and cellular and humoral responses are active defense mechanisms. These responses are regulated by signaling pathways that are activated to cause the expression of genes in charge of managing different defense mechanisms, one of which is the Toll pathway, which has been conserved throughout evolution. The recognition of pathogen-associated molecular patterns (PAMPs), which are β-1,3 glucans in fungi and peptidoglycans in bacteria and are recognized by GNBPs or βGRPs, activates this pathway in larvae and adults. These proteins are distinguished by having a non-functional β-1,3 glucanase GH16 domain and an N-terminal carbohydrate-binding domain known as CBM that will signal the presence of the pathogen, triggering the response to combat it. Furthermore, many insects have functional β-1,3 glucanases with only the GH16 domain. In Lepidoptera, all orthologs of GNBPs (the βGRPs) are able to recognize both gram-positive and negative bacteria as well as fungi, in contrast to Drosophila, where GNBP1 recognizes peptidoglycans (PGs) from gram-positive bacteria and GNBP3 recognizes β-1,3 glucans from fungi. However, non-pathogenic or opportunistic microorganisms were used in the majority of studies in this field. Therefore, research is required to determine how these immune system pathways respond to entomopathogen infection, especially in the early stages of infection. We used the sugarcane borer, Diatraea saccharalis, as a study model and looked at genes with the GH16 domain (βGRPs and β-1,3 glucanases). Thus, in third instar caterpillars exposed to sublethal doses of the pathogens Metarhizium anisopliae and Bacillus thuringiensis, the expression of the identified genes was assessed. Additionally, in caterpillars fed various diets, the expression of a gene encoding a β-1,3 glucanase was assessed. Finally, we discuss how these immune response-related genes, and their associated genes react to naturally occurring pathogens, which have mechanisms to circumvent insect defenses.

COMMITTEE MEMBERS:
Presidente - JOSE DIJAIR ANTONINO DE SOUZA JUNIOR
Externo à Instituição - FERNANDO CAMPOS DE ASSIS FONSECA - IFGO
Externo à Instituição - GUILHERME DUARTE ROSSI - UNESP