Comparison of Statistical Methods Applied in Repeated Measures Over Time in Forestry Experimentation
Agroforestry System; Eucalyptus; Time Series; MANOVA.
The objective of this research was to evaluate the height growth of forest species, cultivated in an agroforestry system, including two clones of Eucalyptus (Eucalytpus urophylla × Eucalytpus tereticornis), Aroeira (Myracrodruon urundeuva Allemão) and Angico (Anadenanthera colubrina var. Cebil), in intercrops or monocultures with an agricultural species, Cowpea (Vigna unguiculata (L) Walp.) and a forage species, Tanzânia Grass (Panicum maximum Jacq), and verify the effect of the spacings 3 m × 2 m in monoculture and 4 m × 2 m, both in monocultures and in intercrops with Tanzânia Grass and Cowpea, and also the effectiveness of the application of nutrient sources and a soil conditioner, residue from fish farming tanks, sediments from Itaparica reservoir, biochar and control, each repeated four times. The experiment is located in the municipality of Belém de São Francisco, Pernambuco, and was implemented in March 2014, at the Experimental Station of the Agronomic Institute of Pernambuco. The experimental design is multivariate with repeated measures over time. The plantings were organized in two spacings 4 m × 2 m for tree species, both in monoculture and in consortium with Cowpea and Tanzânia Grass, and with spacing of 3 m × 2 m for tree species in monoculture. The tree species were allocated to 256 plots, each containing 28 plants, 10 of which occupy the useful area. Plots with 3 m × 2 m spacing are 14 m × 12 m in size, with an area of 168 m² per plot, in 4 m × 2 m spacing they have dimensions of 14 m × 16 m, and an area of 224 m² per plot. To carry out the analysis, three statistical methods were used and compared: multivariate with repeated measurements over time, split-plot design and time-series analysis. The 4 m × 2 m spacings combined with Tanzânia Grass and Cowpeas demonstrated promising results, being the most favorable spacing options. The nutrient sources did not show statistical differences between them. The MA 2000 and MA 2001 clones stood out as the species with the greatest growth, however, the MA 2001 clone presented the highest heights in practically all periods analyzed, statistically differentiating itself from all other species. The MA 2001 clone appears to be a highly promising option for cultivation in the region, showing accelerated growth and proving to be suitable to meet local energy demands. The results highlight the relevance of choosing fast-growing species to meet energy needs, especially in semi-arid regions, where illegal logging of native species is a concern. Furthermore, this approach offers opportunities to diversify the sources of income for farmers in the semi-arid region, reducing exclusive dependence on conventional agriculture, since eucalyptus wood has significant value and is used in several sectors.