Banca de DEFESA: FABIO JOSE RIBEIRO SIMAS

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : FABIO JOSE RIBEIRO SIMAS
DATE: 26/02/2025
TIME: 08:30
LOCAL: DZ
TITLE:

PRODUCTIVITY AND SPERM QUALITY OF BOARS SUPPLEMENTED WITH INCREASING LEVELS OF L-GLUTAMINE AND L-GLUTAMIC ACID

KEY WORDS:

AMINOGUT

PAGES: 101
BIG AREA: Ciências Agrárias
AREA: Zootecnia
SUBÁREA: Produção Animal
SPECIALTY: Criação de Animais
SUMMARY:

The evaluation of swine semen is an efficient tool in the production of quality insemination doses and for monitoring the productive and reproductive performance of the boar. Diets that provide improvements in ejaculate are still a desire in modern swine farming. The amino acids L-glutamine and L-glutamic acid have potential to compose diets, since they act beneficially on the body. The objective of this study was to evaluate the parameters of productivity and sperm quality of fresh semen, durability and sperm quality of cooled semen and sperm quality of post-thawed semen of 24 boars with an average age of (21.5 ± 4.40 months) supplemented with 0.0%, 0.5%, 1.0% of AminoGut (Gln and Glu). For 120 days, the animals were housed and distributed in a randomized block design (DBC) with 3 treatments (0.0%, 0.5%, 1.0% AminoGut), 4 replicates (Blocks) with 2 boars each. Daily, 2.5 kg of standard central feed (RPC) was provided to the animals and the supplement provided un top. To evaluate testicular biometry, measurements (CTD, LTD and CTE LTE) were taken with the aid of a caliper, performed on (M1 = 15th day and M2 = 115th day). To evaluate sperm productivity and quality, the rich fraction of the ejaculates was collected twice a week and evaluated in a CASA (Computer-Assisted Semen Analysis) system of (VE; CSptz; CSptzV; M; P; Sptz bi/C; Sptz bi/M) and ND 2x109). In the evaluation of sperm morphology, using the stained smear test, the following parameters were evaluated: (SptzN; TP; DC; DA; DPI; CDE; CDGCP; CDGCD; GCP; GCD and CI) and in the supra vital test (V and M). In the evaluation of cooled semen, every 40 days (C1 = 40, C2 = 80 and C3 = 120), after collection, the sperm-rich fraction of the ejaculates was diluted (1:1; v/v) in the cooling extender and packaged (80 mL sachet) and evaluated for durability (D1 = 24h and D5 = 120h) and sperm quality (LA, LMP and EOS) in flow cytometry. For the evaluation of post-thawed semen, 3 collections were performed (C1=92, C2=99 and C3=106 days), and the sperm-rich fraction was diluted (1:1; v/v) in the cooling extender to obtain the concentration for freezing. After thawing, the parameters (MT; MP, ALH; BCF; DAP; DCL; DSL; LIN; STR; VAP; VCL; VSL and WOB) were evaluated in the CASA system and by flow cytometry (LMP; LA; PMM and IC). In the results of testicular morphology, there was a statistical difference (p<0.05) between the lineage (D) and the others (A, B and C) and there was a statistical difference (p<0.05) between the measurements (M1= 15 and M2= 115 days). In sperm productivity and quality, there was a statistical difference (p<0.05) between treatments, with (0.5% and 1.0%) outperforming the control (0.0%), among strains, with emphasis on strain (B) and between sperm cycles. In sperm morphology, there was a statistical difference (p<0.05) between strains, with results alternating between them. In cooled semen, there was a statistical difference (p<0.05) when comparing the 3 collections, regardless of treatments, for (EOS). In the evaluation of semen durability, there was a statistical difference (p<0.05) with an increase in LA and LMP between times (D1= 24h and D5= 120h), regardless of treatments. For post-thawed semen, there was no effect of treatment (p>0.05) on the kinetic parameters evaluated, as well as on the parameters of (LA, LMP, EOS and IC). In short, the supplementation of 0.5% and 1.0% L-gln and L-glu for boars provided a positive effect on the productivity parameters of fresh semen, but not on cooled and post-thawed semen, thus indicating positive metabolic effects for this animal category. These results show the need for continued research to obtain better results in pig reproduction through the use of cooled semen and post-thawed semen.

COMMITTEE MEMBERS:
Presidente - HELENA EMILIA CAVALCANTI DA COSTA CORDEIRO MANSO
Interna - CAMILLA MENDONCA SILVA
Interna - LILIAN FRANCISCO ARANTES DE SOUZA
Externo ao Programa - 2150891 - HELIO CORDEIRO MANSO FILHO - UFRPEExterna à Instituição - MARIANA GROKE MARQUES - EMBRAPA