Banca de QUALIFICAÇÃO: LILIANE PEREIRA SANTANA

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : LILIANE PEREIRA SANTANA
DATE: 03/06/2024
TIME: 14:00
LOCAL: Google meet
TITLE:

PROSPECTION, ISOLATION, IDENTIFICATION AND CHARACTERIZATION OF LACTIC BACTERIA AND THEIR USE AS AN INOCULANT IN PEAR MILLET SILAGE

KEY WORDS:

Silage, pH, productivity, nutritional value, semi-arid

PAGES: 90
BIG AREA: Ciências Agrárias
AREA: Zootecnia
SUBÁREA: Pastagem e Forragicultura
SPECIALTY: Avaliação, Produção e Conservação de Forragens
SUMMARY:

The objective of this proposal is to prospect, isolate, identify and characterize lactic acid bacteria and their use as an inoculant in millet ensiling. Initially, millet will be ensiled, where colonies of lactic acid cultures will be isolated from the plant and silage during fermentation periods: 3, 10, 30, 60 and 120 days. During these same periods, the populations of lactic acid bacteria, molds, yeasts and enterobacteria will be quantified, as well as the fermentative profile of the silages will be evaluated. After the colonies are isolated and purified, gram staining tests, catalase reaction, growth at different temperatures, salt concentrations and pH will be carried out. The antagonist activity of the isolates will also be monitored. The isolates will be identified through DNA extraction and amplification using the PCR technique. The sequences obtained from each isolate will be compared with those available in the GenBank database, and aligned using the BLASTn (Basic Local Alignment Search Tool) algorithm (http://www.ncbi.nlm.nih.gov/BLAST) for nucleotides . The 16S rRNA gene sequences that showed similarity equal to or greater than 97% will be considered as belonging to the same Operational Taxonomic Unit (UTO). In the second stage, the pre-selected isolates will be used in an experiment with the objective of evaluating the effect of inoculants isolated during ensiling on the fermentative profile, microbial populations, losses, aerobic stability and chemical composition of millet silage. The experimental design will be completely randomized, arranged in a 5 × 5 factorial scheme, with 5 treatments and 5 opening periods (3, 7, 15, 45 and 90 days after ensiling), with 5 replications. Of the treatments, three will be the lactic acid bacteria strains isolated from experiment 1, based on antimicrobial activity and the results of biochemical tests. Therefore, the treatments will be: Control – without inoculant; Homofermentative lactic acid bacteria; Heterofermentative lactic acid bacteria; Mix of homofermentative lactic acid bacteria and heterofermentative lactic acid bacteria; and a commercial inoculant for corn silage. By carrying out this research, we hope to understand the millet ensiling process and reveal lactic cultures with the potential to be used as an inoculant.

COMMITTEE MEMBERS:
Presidente - ALEXANDRE CARNEIRO LEAO DE MELLO
Interno - FRANCISCO FERNANDO RAMOS DE CARVALHO
Interno - MARCIO VIEIRA DA CUNHA
Externo à Instituição - TADEU VINHAS VOLTOLINI
Externo à Instituição - RICARDO LOIOLA EDVAN - UFPI