Banca de DEFESA: CAROLINA LOUISE NASCIMENTO DE SANTANA

BROMATOLOGICAL, MICROBIOLOGICAL EVALUATION AND LIPID OXIDATION OF NATURAL FEEDS FOR DOGS AND CATS SOLD IN RECIFE-PE

microbiology, nutrition of dogs and cats, bromatology, natural diets.

Given the growth of the natural food market for companion animals, the present work
aimed to carry out bromatological, microbiological and lipid oxidation analyzes on seven
natural food brands that sell raw and cooked natural diets for dogs and cats in Recife –
PE. The bromatological analysis of Dry Matter, Moisture, ether extract, crude protein,
mineral matter and crude fiber were carried out using the methodology described by Silva
and Queiroz (2009). For microbiological analyzes, identification and counting of aerobic
mesophilic microorganisms, Salmonella spp., Staphylococcus aureus, Escherichia coli,
filamentous fungi and yeasts were carried out using the methodology of the standards that
deal with the microbiology of food and feed in the 10th edition of the ABINPET PET
FOOD manual . For lipid oxidation analysis, the TBARS test was performed to identify
lipid oxidation and possible food spoilage. After obtaining the data, the normality of the
data was assessed using the Shapiro Wilk test and the means were compared using the t
test for non-dependent samples using the Bio Estat 5.3 software. The results obtained for
bromatological analysis of natural diets sold by companies with and without registration
in MAPA, where there was a significant difference (p<0.05) between the average
humidity (company with an average registration of 80.55% in MS and company without
registration of 74.45% in MS) and mineral matter (company with 4.5% registration in MS
and company without registration of 3.5% in MS). And for raw and cooked natural diets,
there was a significant difference (p<0.05) between the averages of mineral matter (raw
diet with a value of 6.49% in DM and for cooked diet 3.76% in DM), and ether extract
(18% raw diet in DM and 7.6% cooked diet in DM). In the microbiological analysis,
natural diets sold by companies registered and without registration with MAPA showed
a significant difference (p<0.05) between the averages of aerobic mesophilic
microorganisms (company with registration value of 3.5x106 CFU/25g and company
without registration 4.6 x 106 UFC/25g), E. coli (company with a registered value of 1.1
x 105 UFC/25g and a company without a registration of 2.2 x 105 UFC/25g) and
Staphylococcus aureus (company with registration 8. 5x104 UFC/25g and unregistered
4.7x104 UFC/25g). In the microbiological analysis of raw and cooked natural diets, there
was a significant difference (p<0.05) between the averages of mesophilic aerobic
microorganisms (raw diet - 4.8x106 CFU/25g and cooked diet - 3.7x106 CFU/25g) and
E. coli (raw diet -1.8x105 CFU/25g and cooked diet 9.7x104 CFU/25g). For lipid
oxidation tests of natural diets sold in Recife-PE by companies with and without MAPA
registrations, there were significant differences (p<0.05) between the average TBARS
values for natural diets sold by companies with and without record. Where natural diets
from unregistered companies presented higher average TBARS values (1.2 mg
mannonaldehyde/kg) than natural diets sold by registered companies (0.7 mg
mannonaldehyde/kg). From the lipid oxidation tests of raw and cooked natural diets, there
were significant differences (p<0.05) between the mean TBARS values for the raw (0.15
mg mannonaldehyde/kg) and cooked (1.3 mg mannonaldehyde) natural diets. /kg).
Therefore, despite the increased market interest in Brazil in offering a more natural,
healthy food for dogs and cats, there is no point if these foods do not meet the nutritional
requirements for dogs and cats and the minimum food safety for animal consumption.