Banca de DEFESA: GUILHERME VALERIANO SILVA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : GUILHERME VALERIANO SILVA
DATE: 24/02/2025
TIME: 08:00
LOCAL: https://meet.google.com/bsg-wqio-oan
TITLE:

Occurrence of Extended Spectrum Betalactamase (ESBL) Enterobacteriaceae in milk and feces of dairy goats in the state of Pernambuco, Brazil

KEY WORDS:

Bacterial resistance. Caprine mastitis. Enterobacteriaceae. ESBL. Production animals.

PAGES: 54
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUMMARY:

Enterobacteria are widely dispersed agents in nature, found in soil, water and plants, as well as colonizing the gastrointestinal tract of animals and humans. Members of this family have a great capacity for genetic mutation and this characteristic facilitates the production and dissemination of resistance genes and virulence factors. The aim of this study was to identify Enterobacteriaceae present in the milk and feces of dairy goats and kids in herds in the state of Pernambuco and to evaluate their phenotypic profile for sensitivity to antimicrobials and genotypic profile for the expression of genes coding for antimicrobial resistance and virulence factors. Rectal swab and milk samples were collected from 56 goats and 19 rectal swab samples from kids. The phenotypic sensitivity profile to antimicrobials was identified by the agar disk diffusion method using antibiotic disks, Ceftazidime, Ceftriaxone, Cefotaxime and Aztreonam, followed by the confirmatory Extended Spectrum Beta-Lactamase (ESBL) Double Disk Synergy Test Genotypic profiling for the ESBL-encoding genes was carried out for the target genes blaSHV, blaTEM and blaCTX-M, and for the virulence factor-encoding genes the target genes stx1, stx2, eaeA and hlyA were used. The phenotypic test identified that 63% (45/71) and 28% (6/21) of the isolates from goats and young goats, respectively, were resistant and that 27% (25/92) of the total isolates were ESBL positive. In the genotypic tests to identify genes encoding ESBL, one isolate was positive for blaSHV and one for blaTEM, and for genes encoding virulence factors, 32% (30/92) of the isolates were positive, with no isolates identified as positive for blaCTX-M and eaeA. The identification of enterobacteria resistant to antimicrobials and producing virulence factors in healthy goat herds contributes to the epidemiological characterization of these agents in the rural environment, characterizing them as possible contaminants of animal products.

COMMITTEE MEMBERS:
Presidente - RINALDO APARECIDO MOTA
Externo ao Programa - 1652122 - JOSE WILTON PINHEIRO JUNIOR - UFRPEExterna à Instituição - POLLYANNE RAYSA FERNANDES DE OLIVEIRA