Banca de DEFESA: APOLÔNIA AGNES VILAR DE CARVALHO BULHÕES

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : APOLÔNIA AGNES VILAR DE CARVALHO BULHÕES
DATE: 11/10/2024
TIME: 09:00
LOCAL: VIDEOCPONFERÊNCIA
TITLE:

STUDY OF THE HEALING ACTIVITY OF BASIL ESSENTIAL OIL (Ocimum basilicum L.) IN SKIN WOUNDS OF RATS

KEY WORDS:

Healing, phytotherapy, Ocimum, skin.

PAGES: 73
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Clínica e Cirurgia Animal
SUMMARY:

Ocimum basilicum L. is popularly known as basil, basil and basil, native to tropical Asia, rich in
essential oil that mainly has anti-inflammatory and antimicrobial activity. The objective of this
work was to evaluate the healing activity of basil essential oil (Ocimum basilicum L.) on skin
wounds in rats. The leaves were collected at the DoisIrmãos Campus of the Federal Rural
University of Pernambuco (UFRPE). The oil was extracted using the hydrodistillation technique,
using the Clevenger apparatus and analyzed by gas chromatography coupled to mass
spectroscopy (GC/MS), subsequently, the preparation of an ointment. For the healing activity, 30
albino Wistar rats (Rattus norvergicus albinus) were used, distributed into two experimental
groups of 15 animals each (GT – animals with wounds treated with ointment containing 10%
Ocimum basilicum essential oil; CG – animals with wounds treated with base ointment),
subdivided into 3 groups of 5 animals each, corresponding to postoperative times (PO): 3, 7 and
14 days. Four surgical wounds were made in each animal, in the right and left dorsolateral
thoracic region, with the aid of an 8 mm diameter punch, treated daily and evaluated at 3, 7 and
14 days PO, regarding the degree of contraction and evaluation. macroscopic, morphological and
morphometric regarding the quantification of blood vessels. The phytochemical study revealed
the presence of terpenes and obtained 0.63% essential oil yield. Regarding the degree of wound
contraction, there was no statistical difference between the groups tested. Macroscopically, the
wounds in the treated group showed less inflammation, hyperemia and secretion, more developed granulation tissue, formation of a lighter colored low crust and the lesion was less firm, when compared to the control group. The quantification of blood vessels was significantly higher in the treated group, when compared to the control group, on day 7 PO (p=0.04). In the morphological evaluation, the GT showed a lower concentration of inflammatory cells, greater and better filling of the granulation tissue by collagen fibers, less crust formation, a greater concentration of newly formed vessels and greater deposition of extracellular matrix. It is concluded that the ointment based on essential oil from the leaves of Ocimum basilicum L. at 10% proved to be efficient in the healing process of skin wounds in rats.

COMMITTEE MEMBERS:
Interno - FABRICIO BEZERRA DE SA
Presidente - JOAQUIM EVENCIO NETO
Externa à Instituição - LIGIA REIS DE MOURA ESTEVÃO
Externa à Instituição - LIRIANE BARATELLA EVENCIO - UFPE
Externo à Instituição - MANUEL DE JESUS SIMÕES - USP