Banca de DEFESA: RENAN HENRIQUE DOS SANTOS FAGUNDES

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : RENAN HENRIQUE DOS SANTOS FAGUNDES
DATE: 30/08/2024
TIME: 15:00
LOCAL: Programa de Pós-Graduação em Medicina Veterinária
TITLE:

Proteomic Evaluation of Follicular Fluid and In Vitro-Produced and Vitrified Goat and Sheep Embryos

 

KEY WORDS:

Fertility; Biomarker; Protein; Capra hircus; Ovis aries.

PAGES: 105
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Reprodução Animal
SUMMARY:

This thesis is composed of three chapters on different themes related to the use of proteomics for evaluating reproductive aspects of goats and sheep. The first chapter presents a review of the role of proteomics in assisted reproduction in small ruminants, focusing on goats and sheep. The importance of follicular fluid as a critical environment for oocyte maturation and embryonic development is highlighted, being composed of proteins, hormones, and other essential biochemical factors. The review explores how proteomics allows the identification of biomarkers for the selection of oocytes and embryos, optimizing assisted reproduction protocols. Studies indicate that differences in the proteomic profile between species can influence oocyte competence and reproductive outcomes, reinforcing the need for species-specific approaches. The second chapter presents a comparative analysis of the follicular fluid of goats (Capra hircus) and sheep (Ovis aries), aiming to identify biomarkers of oocyte competence and embryonic development. Significant differences were observed between the species, with goats showing a higher presence of proteins linked to coagulation and healing, while sheep exhibited proteins related to immune response. The analysis of protein-protein interaction networks revealed distinct molecular pathways in each species, highlighting the need for species-specific assisted reproduction protocols. The third chapter addresses the proteomic analysis of embryos produced in vitro and subjected to vitrification, detailing the impact of this process on the proteomic profile and embryo viability. Critical proteins associated with oxidative stress and cellular homeostasis were identified, with specific differences between species. The research emphasizes the need for additional studies to characterize unclassified proteins, aiming to optimize vitrification and improve assisted reproduction programs.

COMMITTEE MEMBERS:
Interno - ***.302.674-** - MARCOS ANTONIO LEMOS DE OLIVEIRA - UFRPE
Externa à Instituição - FABIANA APARECIDA CAVALCANTE SILVA - UFPE
Externo à Instituição - FELIPE AUGUSTO BOUDOUX MARTINS SALES
Externa à Instituição - JOANE ISIS TRAVASSOS VIEIRA
Externo à Instituição - JOSÉ CARLOS FERREIRA DA SILVA - UFRPE