Banca de DEFESA: LUCAS CARVALHO PEREIRA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : LUCAS CARVALHO PEREIRA
DATE: 30/08/2024
TIME: 12:00
LOCAL: Programa de Pós-Graduação em Medicina Veterinária
TITLE:

Influence of Vitrification on the Proteome of In Vitro-Produced Bovine Embryos

KEY WORDS:

Cattle; Reproduction; Cryopreservation; Proteomics; Embryos.

PAGES: 101
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUMMARY:

This thesis is composed of two chapters, each exploring different aspects of cryopreservation and proteomic analysis in in vitro-produced bovine embryos. The first chapter evaluates the impact of vitrification on embryo morphology and proteomic profile. Fresh and vitrified embryos were analyzed, showing a significant reduction in the number of blastomeres in vitrified embryos, associated with alterations in the proteomic profile, particularly in proteins related to cellular stress. These results indicate that vitrification compromises embryonic viability, highlighting the need to improve cryopreservation protocols to enhance outcomes in assisted reproduction. The second chapter compares the viability and proteomic profile of embryos subjected to vitrification and direct transfer. Four groups of embryos were analyzed, focusing on proteins involved in cell stability and stress response. Vitrified embryos that maintained development until re-expansion and those subjected to direct transfer showed higher viability, suggesting the importance of additional culture for vitrified embryos and highlighting direct transfer as an effective alternative to vitrification. These studies provide valuable insights for optimizing cryopreservation protocols in assisted reproduction programs, focusing on proteomic approaches to improve embryo selection and viability.

COMMITTEE MEMBERS:
Interno - ***.302.674-** - MARCOS ANTONIO LEMOS DE OLIVEIRA - UFRPE
Externa à Instituição - FABIANA APARECIDA CAVALCANTE SILVA - UFPE
Externo à Instituição - FELIPE AUGUSTO BOUDOUX MARTINS SALES
Externa à Instituição - JOANE ISIS TRAVASSOS VIEIRA
Externo à Instituição - JOSÉ CARLOS FERREIRA DA SILVA - UFRPE