Banca de DEFESA: JÉSSICA CRISTIANNE MAZER BERNARDI

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : JÉSSICA CRISTIANNE MAZER BERNARDI
DATE: 29/08/2024
TIME: 14:00
LOCAL: Google Meet
TITLE:

Epidemiology and molecular characterization of hemoparasites in cats domestic birds (Felis catus) in the state of Pernambuco, Brazil.

KEY WORDS:

diagnosis; feline mycoplasmosis; felines; leishmaniasis; PCR.

PAGES: 80
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Patologia Animal
SPECIALTY: Patologia Clínica Animal
SUMMARY:

With the increase in the acquisition of cats as companion animals and their frequent visits to public areas, there has been a rise in the number of people exposed to the risk of parasitic infections. This is due to the fact that cats harbor various zoonotic agents, which can be transmitted through different routes. Although emerging diseases caused by hemoparasites in companion animals have been investigated in recent years, there are few reports of epidemiological and molecular studies characterizing these pathogens in domestic cats. Therefore, the aim of this study was to conduct an epidemiological survey and molecular characterization of gram-negative bacteria such as Ehrlichia, Mycoplasma, and protozoa of the genera Babesia and Leishmania sp. in domestic cats in the state of Pernambuco. For this purpose, blood samples were collected via cephalic, jugular, or lateral saphenous venipuncture, and DNA was extracted from 200 μl of blood using the Purelink® Genomic DNA Kits (Invitrogen® by Life Technologies®), following the manufacturer's instructions. PCR was performed using specific primers for each targeted agent, and the amplification products were subjected to 1.5% agarose gel electrophoresis, stained with ethidium bromide (0.5 µg/mL). Of the 124 samples analyzed, 36 (29%) were positive for Mycoplasma haemofelis, 1 (0.8%) was positive for Leishmania infantum and no sample was positive for Babesia sp. and Ehrlichia sp. through molecular diagnosis. For better understanding, this thesis qualification work was divided into a literature review and two chapters, namely: Chapter 1 "Prevalence and risk factors associated with Mycoplasma haemofelis infection in domestic cats in the metropolitan region of Recife, Pernambuco, Brazil"; Chapter 2 " Molecular and parasitological detection of Leishmania sp. in peripheral blood of domestic cats from the state of Pernambuco, Brazil – report of three cases."

COMMITTEE MEMBERS:
Presidente - FABIO DE SOUZA MENDONCA
Interno - JOAQUIM EVENCIO NETO
Externo à Instituição - LUCIANO SANTOS DA FONSECA
Externa à Instituição - TELMA DE SOUSA LIMA - UECE
Externo à Instituição - VICTOR FERNANDO SANTANA LIMA
Externa à Instituição - JULIANA FORTES VILARINHO BRAGA
Externa à Instituição - JESSICA DE TORRES BANDEIRA