Banca de QUALIFICAÇÃO: JÉSSICA CRISTIANNE MAZER BERNARDI

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : JÉSSICA CRISTIANNE MAZER BERNARDI
DATE: 05/08/2024
TIME: 14:00
LOCAL: Google Meet
TITLE:

Epidemiology and molecular characterization of hemoparasites in cats domestic birds (Felis catus) in the state of Pernambuco, Brazil.

KEY WORDS:

diagnosis; feline mycoplasmosis; felines; leishmaniasis; PCR.

PAGES: 80
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Patologia Animal
SPECIALTY: Patologia Clínica Animal
SUMMARY:

With the increase in the acquisition of cats as companion animals and their frequent
visits to public areas, there has been a rise in the number of people exposed to the risk
of parasitic infections. This is due to the fact that cats harbor various zoonotic agents,
which can be transmitted through different routes. Although emerging diseases caused
by hemoparasites in companion animals have been investigated in recent years, there
are few reports of epidemiological and molecular studies characterizing these pathogens
in domestic cats. Therefore, the aim of this study was to conduct an epidemiological
survey and molecular characterization of gram-negative bacteria such as Ehrlichia,
Mycoplasma, and protozoa of the genera Babesia and Leishmania sp. in domestic cats
in the state of Pernambuco. For this purpose, blood samples were collected via cephalic,

jugular, or lateral saphenous venipuncture, and DNA was extracted from 200 μl of

blood using the Purelink® Genomic DNA Kits (Invitrogen® by Life Technologies®),
following the manufacturer's instructions. PCR was performed using specific primers
for each targeted agent, and the amplification products were subjected to 1.5% agarose
gel electrophoresis, stained with ethidium bromide (0.5 µg/mL). For better
understanding, this thesis qualification work was divided into a literature review and
two chapters, namely: Chapter 1 "Prevalence and risk factors associated with
Mycoplasma haemofelis infection in domestic cats in the metropolitan region of Recife,
Pernambuco, Brazil"; Chapter 2 " Molecular and parasitological detection of
Leishmania sp. in peripheral blood of domestic cats from the state of Pernambuco,
Brazil – report of three cases."

COMMITTEE MEMBERS:
Presidente - FABIO DE SOUZA MENDONCA
Externa à Instituição - JESSICA DE TORRES BANDEIRA
Externa à Instituição - JULIANA FORTES VILARINHO BRAGA