Banca de DEFESA: MARIA SHEILA DA SILVA FERREIRA
Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.STUDENT : MARIA SHEILA DA SILVA FERREIRA
DATE: 28/06/2024
TIME: 08:30
LOCAL: Auditório Luis de Melo Amorim
TITLE:
EXPERIMENTAL SKIN WOUNDS OF DOGS (Canis familiaris) TREATED WITH ROSEMARY OIL (Rosmarinus Officinalis L.) OZONIZED
KEY WORDS:
Essential oil; Neovascularization; Ozone Therapy; Healing.
PAGES: 74
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Clínica e Cirurgia Animal
SUMMARY:
In Brazil, medicinal plants are widely used in Veterinary Medicine to aid in the wound healing process. One such plant is rosemary (Rosmarinus officinalis L.), considered sacred by ancient peoples. Another important tool employed for this purpose is ozone gas in the formulation of creams and oils. When a substance undergoes ozone processing, its effects are potentiated, leading to what is now known as ozone therapy. This experiment aimed to perform a morphometric and histopathological evaluation of surgically created wounds in dogs (Canis familiaris) healing by secondary intention and treated with ozonized rosemary oil. Surgical wounds were created using an 8mm punch, with each animal serving as both control and treatment simultaneously (two wounds per animal). Twenty dogs aged between one and four years, weighing between seven and 20 kg, were divided into four groups (G3, G7, G14, and G21), each containing five animals (n=5). Wounds were evaluated on days three (D3), seven (D7), 14 (D14), and 21 (D21) postoperatively, respectively. Each animal was represented by two subgroups: the control group (CG, treated with 0.9% saline solution) and the treated group (GT, treated with ozonized rosemary oil). This procedure was repeated every 24 hours for 21 days, during which all animals were clinically assessed. On days D3, D7, D14, and D21, lesions from all animals in each subgroup were biopsied, with an incision made in an ellipse encompassing intact skin and all healing tissue. After sample collection, they were stained with Hematoxylin-Eosin for histopathological examination with morphological and morphometric assessments. The data obtained were subjected to the Kruskal-Wallis test and complemented by the Tukey-Kramer test (p<0.05). The wound healing effect of ozonized rosemary oil was observed in wound contraction (p=0.05) in GT compared to CG on D3, and in angiogenesis (p=0.001) in GT compared to CG on D7 and D14. Morphologically, there was less inflammation in GT on D3, D14, and D21, and less crust formation on D3 and D21 compared to CG, although there was no statistical difference between them. It is concluded that the combination of rosemary essential oil with ozone therapy favored the process of wound healing by secondary intention in canine cutaneous wounds.
COMMITTEE MEMBERS:
Externa à Instituição - ANA KARINA RODRIGUES DA MOTA
Interna - GRAZIELLE ANAHY DE SOUSA ALEIXO CAVALCANTI
Presidente - JOAQUIM EVENCIO NETO
Externa ao Programa - 2309518 - LILIAN SABRINA SILVESTRE DE ANDRADE - nullExterna à Instituição - MARIA CRISTINA DE OLIVEIRA CARDOSO COELHO