Banca de QUALIFICAÇÃO: LEANDRO LAMARTINE LOPES ROCHA

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : LEANDRO LAMARTINE LOPES ROCHA
DATE: 29/05/2024
TIME: 14:00
LOCAL: https://meet.google.com/txb-qgqj-zea
TITLE:

Research and characterization of mammary papillomas in goats (Capra aegagrus hircus) raised in the Northeast region of Brazil

KEY WORDS:

histopathological, breast neoplasms, papillomatosis, papillomavirus, health.

PAGES: 112
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Clínica e Cirurgia Animal
SPECIALTY: Clínica Veterinária
SUMMARY:

The present work aims to investigate and characterize the papillomatosis that affects dairy goats located in the Northeast region of Brazil, between the states of Rio Grande do Norte (RN), Paraíba (PB) and Pernambuco (PE), covering the identification and viral typing through PCR followed by genetic sequencing. Visits were made to dairy goat breeding properties in order to inspect the animals until 1000 (one thousand) heads were reached in order to identify possible lesions compatible with papillomatosis. On each property, an investigative questionnaire was applied, covering data on physical structure, number of animals and health management. In addition, containing specific information about the presence of disease in the goat herd and other species. An individual clinical form was filled out for each animal collected with papillomas. Biological material was collected from these animals. Being a fragment of tissue from the lesion using Punch (0.8 mm) fixed in formaldehyde for histopathology. And another sample with papillomas collected by plucking, stored in sterile tubes and frozen for viral identification through PCR and genetic sequencing, respecting ethical principles during and after collection. In total, 57 breeders participated in the study in 13 municipalities in RN, PB and PE. Therefore, 1885 goats of different breeds, age and sex were inspected. 21 goats (1.11%), belonging to 9 breeders, were identified with lesions characteristic of papillomatosis in the mammary glands. Among the 21 animals collected, 38% (8) belonged to the municipality of Sanharó (PE) and 38% (8) belonged to the municipality of Prata (PB), the rest were collected in the following municipalities: 9.5% (2) in Sertânia ( PE), 4.7% (1) in Recife (PE), 4.7% (1) in Monte Alegre (RN) and 4.7% (1) in Ceará-Mirim (RN). Of the 21 animals, 76.1% (16/21) are of the Saanen breed. While 9.5% (2/21) are of the Toggenburg breed, 4.7% (1/21) of the Pardo Alpina breed, 4.7% (1/21) of the American Alpine breed and finally 4.7% (1/21) Mixed race (no defined race). Furthermore, it was observed that all animals with injuries had a dairy capacity of 90.4% (19/21) or a mixed capacity of 9.5% (2/21). 20 animals were macroscopically evaluated. For better understanding and discussion, the papillomas found were classified according to their characteristics: typical, atypical, embedded atypical, filamentous, pedunculated and mixed (when presenting more than one type). As a result, 33.3% (7/21) presented an atypical characteristic; 19% (4/21) typical characteristic; 14.28% (3/21) atypical characteristic embedded; 9.52% (2/21) filamentous characteristic; 4.7% (1/21) presented mixed characteristics (filamentous + pedunculated). Regarding the spread of papillomatosis lesions in the mammary gland, mild intensity prevailed, with 71.4% (15/21). Followed by an intense degree with 23.8% (5/21) and 4.7% (1/21) moderate. The color of the most commonly found papillomas was brown, with 81% (17/21), followed by gray color and skin color with 9.5% (2/21) each. We found in our study that in addition to the lesions characteristic of papillomatosis, some goats also presented another type of lesion in the mammary glands characterized as exuberant neoplastic formations (FNE), being present in 19% (4/21) of the animals. Only 20 animals were evaluated microscopically. The formalin-fixed samples were processed in the Pathology sector at UFRPE. Histopathological findings demonstrated keratinization in all samples. The intensity of its presence is represented by + (mild), ++ (moderate) and +++ (intense). Therefore, 50% (10/20) of them presented greater intensity (+++), 30% (6/20) with lower intensity and 20% (4/20) with moderate intensity. We understand that the lesions evaluated present keratinization, presence of inflammatory cells (lymphoplasmacytic), epidermal hyperplasia, hyperkeratosis and hypergranulosis. Four animals were evaluated in molecular tests. A sample was processed at the Laboratory of Molecular Studies and Experimental Therapy (LEMTE) at the Federal University of Pernambuco (UFPE), which, through PCR, showed the presence of BPV-2. And three samples were sent to the Genetics laboratory of the Butantan Institute, São Paulo and four viral sequences were identified in DNA samples corresponding to three animals, including two supposed new types of BPV (78% similarity in equivalent amino acid sequence compared to supposed BAPV-5 type and 72% similarity in equivalent amino acid sequence compared to the BPV-7 type), in addition to co-infection with the BPV-5 and BPV-13 types, revealed in the same sample. Therefore, of the 21 animals collected, 20 goats were evaluated macroscopically, 20 goats microscopically and 4 animals underwent molecular analysis. Thus, the work sought to report information that had not yet been studied about papillomatosis in the mammary glands of dairy goats in the Brazilian Northeast. Furthermore, identify the characteristics of macroscopic and microscopic lesions and viral strains that are related to goats in the region.

COMMITTEE MEMBERS:
Presidente - HUBER RIZZO
Externo à Instituição - MÁRIO FELIPE ALVAREZ BALARO - UFF
Externa à Instituição - TATIANE RODRIGUES DA SILVA - UFCG