Banca de DEFESA: ERICA BRUNA DE ANDRADE SOARES

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : ERICA BRUNA DE ANDRADE SOARES
DATE: 13/03/2024
TIME: 14:30
LOCAL: Videoconferência: Link: https://meet.google.com/iux-ieze-qnw
TITLE:

MORPHOLOGICAL, MORPHOMETRIC AND THERMOGRAPHIC EVALUATION OF SKIN WOUNDS IN WISTAR RATS TREATED WITH OINTMENT BASED ON ESSENTIAL OIL FROM LEMON PEEL (Citrus limon)

KEY WORDS:

Herbal medicine, Citrus limon, skin, herbal medicines.

PAGES: 100
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Clínica e Cirurgia Animal
SUMMARY:

The objective of this work was to evaluate morphologically, morphometrically and thermographically the cutaneous wounds in Wistar rats, treated with ointment containing Sicilian lemon essential oil (Citrus limon) at 10%. Forty adults male Wistar rats were used, divided into two groups of 20 animals, namely: Treated Group (TG) wounds treated with an ointment composed of essential oil from the peel of Sicilian lemon fruits at a concentration of 10%, incorporated into Vaseline base and Control Group (CG), wounds treated with ointment containing only vaseline base. Each animal was submitted to two incisions in the skin, in the dorsolateral thoracic region, in the right and left antimeres. The wounds were measured on days 0, 3, 7, 14 and 21 postoperatively using a digital caliper to assess the degree of contraction and analyzed macroscopically. On the 3^rd, 7^th, 14^th and 21^st days of treatment, the animals were anesthetized and samples from the wounds of 5 animals from each group were collected and fixed in neutral buffered formalin at 10%. After fixation, the samples were subjected to routine methodology for impregnation and inclusion in histological paraffin, from which histological sections with a thickness of 5 µm were obtained, which were then stained with Hematoxylin-Eosin (HE) and Gomori's Trichrome, for morphological and morphometric evaluations. The obtained data were submitted to the ANOVA test complemented by the Tukey-Krame test (p<0.05). Macroscopic results indicated a slight difference between treated and control groups, but without statistical significance. In the quantification of blood vessels, there was great statistical significance on TG-14 in relation to CG and thermographic analysis indicated great statistical difference on days 7 and 14 PO. Based on the results found, it is concluded that the essential oil from Citrus limon peel on skin wounds has healing activity, improving the healing process in the treated groups.

COMMITTEE MEMBERS:
Interno - FABRICIO BEZERRA DE SA
Presidente - JOAQUIM EVENCIO NETO
Externa à Instituição - LIRIANE BARATELLA EVENCIO - UFPE
Externo à Instituição - MANUEL DE JESUS SIMÕES - USP
Externa ao Programa - 385042 - MARIA JOSE DE SENA - null