Banca de DEFESA: MARINA CAVALCANTI PEREIRA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : MARINA CAVALCANTI PEREIRA
DATE: 28/02/2024
TIME: 14:30
LOCAL: Videoconferência: Link: https://meet.google.com/iue-endr-pkx
TITLE:

Histological evaluation of the healing effect of different acupuncture techniques for the treatment of excisional wounds in albino Wistar rats (Rattus norvergicus albinus)

KEY WORDS:

Healing by secondary intention; Electroacupuncture; Low Intensity Laser; Reepithelialization; Tissue repair

PAGES: 78
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUMMARY:

This work aimed to evaluate different acupuncture techniques for the treatment of excisional wounds in albino Wistar rats. For this, 100 (one hundred) male rats were selected, divided into 5 groups of 20 animals: GC - no treatment; GA1- Animals treated with systemic acupuncture at points: B13, B17 and E36, GA2- Animals treated with acupuncture, using the cerclage technique; GEA electroacupuncture, using the cerclage technique at a frequency of 2Hz and 1mA output. GL – laser at a wavelength of (660 nm) with a power of 100 mW and a decreasing dose of 5 J/point. All animals received treatments every other day for 21 days. On postoperative days 3, 7, 14 and 21, 5 animals from each group were euthanized to collect samples from the wound area and subsequent observation and microscopic analysis. HE and Gomori Trichrome staining were used and angiogenesis and tissue morphology (type and intensity of cellular infiltrate), degree of re-epithelialization and keratinization, presence and reorganization of collagen and presence of hair follicles and collagen fibers were evaluated. The results demonstrated a difference in wound contraction on the 7th PO day between GA2 and GEA (p < 0.05), GA2 and GL (p < 0.01), GL and GC (p < 0.05), on the 14th PO day , between GA1 and GA2 (p < 0.001), GA2 and GC (p < 0.01), GEA and GC (p < 0.01), GL and GC (p < 0.01) and on the 21st day PO between GA2 and GEA (p < 0.05), GL and GEA (p < 0.001), GEA and GL (p < 0.05). Regarding wound epithelialization, there was a significant difference on day 7 PO (GA1 = GA2 = GL > GEA =GC) and on day 14 PO (GC = GA1 = GA2 = GEA> GL). Regarding crust formation, there was a difference on day 14 (GC > GL > GA2 > GEA = GA1) and on day 21 (GL > GEA > GC = GA1 = GA2). When evaluating the degree of inflammation, differences occurred on day 7 PO (GL = GC = GEA > GA1 = GA2), day 14 PO (GL > GEA = GC = GA1 > GA2) and day 21 PO (GC> GL > GEA > GA1 > GA2). Regarding the degree of extracellular matrix deposition, there was a difference between the groups on days 14 PO and 21 PO (GA1 = GA2 = GEA > GL = GC). After analyzing the experiment, it was observed that the three acupuncture techniques for epithelial healing by secondary intention were effective. As there was no linear result, it is possible that the combination of techniques at different times resulted in a better healing process.

COMMITTEE MEMBERS:
Interno - FABRICIO BEZERRA DE SA
Interna - GRAZIELLE ANAHY DE SOUSA ALEIXO CAVALCANTI
Presidente - JOAQUIM EVENCIO NETO
Externa à Instituição - MARIANA GOMES DO RÊGO
Externo à Instituição - VITOR CAIAFFO BRITO - UFPE