Banca de DEFESA: RAISSA SANTANA RENOVATO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : RAISSA SANTANA RENOVATO
DATE: 27/02/2024
TIME: 13:30
LOCAL: https://meet.google.com/mxp-iqbc-bny
TITLE:

Detection and molecular characterization of Toxoplasma gondii in sheep from slaughterhouses in Pernambuco state, Brazil: Impact on public health.

KEY WORDS:

Bioassay. Epidemiology. PCR, RIFI, Viability.

PAGES: 40
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUMMARY:

Brazil stands out worldwide in the production of foods of animal origin. Sheep farming is of fundamental socioeconomic importance for the Northeast, as the production of these animals represents an alternative to the supply of meat, milk, and derivatives, especially for the rural population. The northeastern herd accounts for approximately 64% of Brazil's sheep, the vast majority of which are exploited by small and medium-sized breeders. In this specific market, consumers are increasingly looking for safe foods, making it essential to invest in the prevention of zoonoses, which can be transmitted through foods of animal origin, with greater focus on toxoplasmosis, a zoonosis with worldwide distribution, caused by the protozoan T. gondii, with felids as its definitive host and more than 300 species of mammals as intermediate hosts, including humans and 30 species of birds. The consumption of raw meat or meat products has been the main route of transmission to humans, with sheep being of greater importance as they present a higher frequency of tissue cysts, causing serious fetal and neonatal disorders in humans, and harm to the health of children, the elderly and immunocompromised adults. Some diagnostic methods for the occurrence of this disease in meat are the ELISA test and PCR. The aim was to study the presence and distribution of Toxoplasma gondii infection in sheep slaughtered in the state of Pernambuco, Brazil, first determining the prevalence of anti-T. gondii antibodies by RIFI and then evaluating the viability of T. gondii in the animals' tissues. by bioassay in mice and from this material, identify the presence of T. gondii DNA by PCR. Samples from 250 sheep were collected on the slaughter line. Blood samples were collected by puncture of the jugular vein. Fragments of liver, heart, brain, lung, and diaphragm were also collected at the  evisceration. The samples were sent to the LDIC at UFRPE. To test for IgG anti-T. gondii, RIFI was used, and the samples with titers greater than or equal to 1:64 being considered positive. Serum-positive tissue samples in the RIFI were macerated in isolation and subjected to the digestion protocol in an acidic pepsin solution to be inoculated into Swiss mice. The inoculated mice were observed for 45 days, before euthanasia and collection of the brain and abdominal lavage, which in turn were subjected to PCR where they were amplified at 529bp. In the present study, 31.64% (81/256) of the sheep were seropositive for anti-T. gondii. Regarding the bioassay in mice (mus musculus) to isolate T. gondii, of the 81 samples inoculated for euthanasia after 45 days, and research for brain cysts and PCR for the genus apicomplexa and toxo – 529. Of the 44 samples analyzed, 32.2% (10/81) of the mice had cysts in the brain, 39.5% (32/44) were positive for the apicomplexan genus and 20.9% (17/44) were positive for toxo 529.

COMMITTEE MEMBERS:
Presidente - RENATA PIMENTEL BANDEIRA DE MELO
Externa à Instituição - THAIS FERREIRA FEITOSA
Externo à Instituição - WAGNNER JOSE NASCIMENTO PORTO