Banca de DEFESA: CAROLINA JONES FERREIRA LIMA DA SILVA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : CAROLINA JONES FERREIRA LIMA DA SILVA
DATE: 23/02/2023
TIME: 16:00
LOCAL: UFRPE - remota
TITLE:

Evaluation of different blood biomarkers in lactating mares and foals Mangalarga Marchador at different stages

KEY WORDS:

 circadian cycle, chronobiology, lactation, metabolic profile.

PAGES: 110
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Clínica e Cirurgia Animal
SPECIALTY: Clínica Veterinária
SUMMARY:

A variety in the results of hematological, biochemical and mineral parameters can result from an extensive diversity of breeds and management, in addition to variables such as age, sex, time of year, nutrition and activity performed; directly influence such parameters. Thus, this study aimed to determine and evaluate hematological, biochemical valuesand mineral profile of lactating mares, suckling foals and yearling fillies in a 12-month period. The experiment was carried out on a farm located in the city of Gravatá-PE (Lat: -8.2096, Long: -35.5695); average annual rainfall: 500 to 1000mm. From a batch of 80 animals, those clinically healthy were selected, submitted to the same nutritional management. Mares and yearling fillies received 2.0 kg/animal/day of concentrate (dry matter: 89.26%; ether extract: 1.66%; crude protein: 8.42%; crude energy: 3794 kcal/kg ; mineral matter: 2.08%; neutral detergent fiber: 32.71%; acid detergent fiber: 22.98%) and with access to Buffel grass (Cenchrus ciliaris cv. Biloela) pasture in the rainy season (March-July) and supplemented with corn silage (3.0-4.0 kg/day/animal in the dry period (August-February), mineralized salt (Co-Equi Tortuga/DSM) and water were provided ad libitum. three distinct groups: lactating mares (16), suckling foals (16) and yearling foals (25). Samples were collected over a period of 12 months and performed through jugular venipuncture in tubes containing EDTA for analysis of hematological parameters and in tubes without anticoagulant to obtain serum biochemical parameters and macromineral profile. The samples were taken to Laboratory B IOPA, at the Department of Animal Science at UFRPE, where they were processed and analyzed. The hematological parameters (red blood cells (HE), hemoglobin (HB), hematocrit (HT), mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (CHCM), distribution range of erythrocytes, standard deviation (RDW-SD), amplitude of distribution of erythrocytes coefficient of variation (RDW-CV), leukocytes (LEU), lymphocytes (LINF), platelets and other white cells) were obtained by counting in automatic hematology equipment (poch-100iV®). Biochemical parameters (serum protein (PS), albumin (ALB), globulin (GLOB), triglycerides (TRIG), very low density lipoprotein (VLDL), low density lipoprotein (LDL), high density lipoprotein (HDL)) were obtained by reading in semiautomatic equipment (Doles®D250) using commercial kits, following the manufacturer's recommendations; the evaluation of minerals (iron (Fe), total calcium (tCa++), ionized calcium (iCa), phosphorus (P), magnesium (Mg++) and chloride (Cl-)) was obtained following the methodology determined for biochemical analysis. The data obtained were submitted to analysis of variance by the ANOVA method, using the Tukey test when necessary, establishing a significance level of 5%.

COMMITTEE MEMBERS:
Externa à Instituição - CLARISSE SIMÕES COLEHO
Presidente - HELIO CORDEIRO MANSO FILHO
Externo à Instituição - JOSE DANTAS RIBEIRO FILHO - UFV
Externa à Instituição - RAISSA KAROLINA SLAGUEIRO CRUZ
Externa à Instituição - SILVIA ROBLES REIS DUARTE