Banca de QUALIFICAÇÃO: FABIANA OLIVEIRA COSTA

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : FABIANA OLIVEIRA COSTA
DATE: 16/12/2022
TIME: 17:00
LOCAL: UFRPE - remota
TITLE:

Evaluation of protein metabolism and oxidative stress in elderly dogs under the influence of glutamine and/or selenium supplementation 

KEY WORDS:

amino acids, antioxidants, catabolism, geriatrics, immunonutrition

PAGES: 87
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Clínica e Cirurgia Animal
SPECIALTY: Clínica Veterinária
SUMMARY:

In aging, all regulatory functions gradually begin to decline and oxidative stress has a major impact on this process. With the progression of age, the bioavailability of nutrients is reduced and the diet has a direct influence. Glutamine (Gln), is an amino acid that can be reduced in catabolic conditions, participating in important functions such as protein synthesis and in the antioxidation system. Selenium (Se) is an essential micromineral, which also plays a significant role in the antioxidant system, and its deficiency in the diet is related to the occurrence of diseases. The aim of this study was to evaluate the effects of supplementation with Gln, Se, and its combined form on Gln metabolism and biomarkers of oxidative stress in elderly dogs. A randomized controlled study was carried out with 30 dogs, regardless of breed or sex, ≥ 10 years old, clinically healthy, without pre-existing condition, using a pre-established inclusion protocol. Three groups were formed: g-GLN; g-SE; g-GLN+SE. The supplements were based on L-glutamine (0.33g/Kg) and selenium chelate (0.006mg/Kg), once a day, for 30 days, and offered in an isolate or combined form. Blood collections occurred before the supplements (T0), and after 15 (T15) and 30 (T30) days after. Behavioral and body condition data (ECC, IMCC) were collected. Serum Gln and glutamate (Glu) levels, gpx activity and lipid peroxidation index by TBARS method, were evaluated. ANOVA was used by the SigmaPlot 13.0 program for Windows® (Systat Software, Inc), and Tukey's test for differences (P≤0.05). The animals presented a pattern of anxious behavior and reduced physical activity; had decreased serum Gln levels at T0, revealing the existence of catabolic events without influencing the clinically healthy state. g-GLN and g-GLN+SE increased serum levels of Gln (P=0.002), demonstrating the importance of this nutrient on late stage of life. Also,there was a reduction in lipid peroxidation, according to results by TBARS method in g-GLN+SE and g-SE (P≤0.001), modulating oxidative stress in this context.

COMMITTEE MEMBERS:
Presidente - HELIO CORDEIRO MANSO FILHO
Externo ao Programa - 2579090 - MOACIR BEZERRA DE ANDRADE - UFRPEExterna à Instituição - ELISAMA DE CASTRO TORRES